PMID: 7536052Feb 1, 1995Paper

Insulin-like growth factor (IGF) binding protein 3 in the rat testis: follicle-stimulating hormone dependence of mRNA expression and inhibition of IGF-I action on cultured Sertoli cells

Biology of Reproduction
M S Rappaport, E P Smith

Abstract

Insulin-like growth factor binding protein 3 (IGFBP-3) is the predominant IGF binding protein produced by cultured rat Sertoli cells. Previous studies have shown that FSH lowers the abundance of IGFBP-3 protein in Sertoli cell culture medium, suggesting that this binding protein has a physiological role in modulating insulin-like growth factor-I (IGF-I) activity in the testis. To characterize the physiological relevance of the FSH regulation of IGFBP-3, in this study we examined effects of FSH on IGFBP-3 mRNA expression in testes from hypophysectomized rats and in Sertoli cell culture. We then examined whether or not IGFBP-3 could alter the effects of IGF-I on cultured Sertoli cells. FSH (1-300 ng/ml) treatment of rat Sertoli cells dose-dependently decreased IGFBP-3 mRNA, with near-complete inhibition by 12 h of treatment. To evaluate this effect in the whole animal, male rats were hypophysectomized at 20 days of age and injected with FSH 10 days later. Testis IGFBP-3 mRNA increased following hypophysectomy relative to the value in sham animals, while FSH treatment decreased IGFBP-3 mRNA to sham levels within 6 h. To determine whether or not IGFBP-3 modulates IGF-I activity in the Sertoli cell, recombinant human non-glycosylate...Continue Reading

Citations

Jul 28, 1999·Molecular Reproduction and Development·M K DyckF Pothier
May 31, 2006·Journal of Molecular Histology·Jale OnerEnver Ozan
Mar 19, 2004·Biology of Reproduction·Varadaraj ChandrashekarAndrzej Bartke
Aug 10, 2004·World Journal of Gastroenterology : WJG·Inma Castilla-CortazarSalvador Gonzalez-Baron
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I Castilla-CortazarJ Prieto
Sep 6, 2005·The Journal of Toxicological Sciences·Tamio FukushimaIkuo Horii
May 2, 2019·Frontiers in Endocrinology·Silvina Beatriz MeroniSelva Beatriz Cigorraga

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