Insulin-like growth factor (IGF) I and retinoic acid induce the synthesis of IGF-binding protein 5 in rat osteoblastic cells

Endocrinology
Y Dong, E Canalis

Abstract

The insulin-like growth factor (IGF) regulatory system has a major impact on bone physiology. Among the modulators of IGFs, a family of structurally related proteins, the IGF-binding proteins (IGFBPs), have been shown to either potentiate or inhibit IGF actions on bone growth. However, the regulation of IGFBP expression in bone cells is not completely understood. In the present study, the expression of IGFBP-5 was analyzed in primary osteoblastic cells (Ob cells) isolated from 22-day-old fetal rat calvariae. Treatment of Ob cells with either IGF-I or all-trans-retinoic acid (RA) caused a time- and dose-dependent increase in IGFBP-5 messenger RNA (mRNA) levels, as determined by Northern blot analysis. Stimulation of IGFBP-5 mRNA was obtained at 100 nM IGF-I between 6 and 16 h (2- to 2.5-fold) and 100 nM RA between 16 and 24 h (3- to 4-fold). Concomitant treatment of Ob cells with IGF-I and RA revealed an additive effect and a 5- to 7-fold increase in IGFBP-5 mRNA levels after 16-24 h. The effect of IGF-I and RA and their combination on IGFBP-5 transcripts was similar in confluent and subconfluent cultures of Ob cells. IGF-I and RA did not change IGFBP-5 mRNA stability in Ob cells after transcription arrest with the RNA polymeras...Continue Reading

Citations

Aug 20, 2008·Breast Cancer Research : BCR·Mustafa AkkiprikWei Zhang
Apr 26, 2008·Endocrine Reviews·Andrea GiustinaErnesto Canalis
Aug 31, 2000·Biochemical and Biophysical Research Communications·Y T HouE M Zimmerman
Nov 9, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Dan Jia, Johan N M Heersche
Mar 27, 2007·Injury·Eleftherios TsiridisPeter Giannoudis
Mar 27, 2007·Injury·Peter GiannoudisGeorge Kontakis
Apr 18, 2006·Injury·Rozalia DimitriouPeter V Giannoudis
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mei ZhangThomas L Clemens
Oct 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anaïs BoglinoManuel Manchado
Apr 24, 2018·Endocrine Reviews·Gherardo MazziottiAndrea Giustina
May 1, 1997·Journal of Cellular Physiology·H L Cheng, E L Feldman
Aug 1, 1997·Journal of Cellular Physiology·B Gabbitas, E Canalis
Nov 5, 1997·Journal of Cellular Biochemistry·S RydzielE Canalis
Dec 8, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ioannis ChristodoulouJulia M Polak
Mar 12, 1998·Endocrine Reviews·T A Guise, G R Mundy
Dec 22, 1999·Endocrine Reviews·V HwaR G Rosenfeld
Nov 14, 1997·The American Journal of Physiology·E M ZimmermannK N Bitar
Jun 19, 2001·American Journal of Physiology. Endocrinology and Metabolism·Z FuH Kato
Mar 11, 2006·The Biochemical Journal·James BeattieDavid J Flint
Jun 16, 1999·Breast Cancer Research and Treatment·M A Oosterlaken-DijksterhuisJ A Mol
Dec 1, 1996·The American Journal of Physiology·C SchmidJ Zapf
Oct 20, 1995·The Journal of Biological Chemistry·C Duan, D R Clemmons

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.