Insulin-like growth factor II receptor, transforming growth factor-beta, and Cdk4 expression and the developmental epigenetics of mouse palate morphogenesis and dysmorphogenesis

Developmental Dynamics : an Official Publication of the American Association of Anatomists
M MelnickT Jaskoll

Abstract

The B10/B10.A congenic mouse pair serves as a model for identifying specific genes related to morphogenesis and dysmorphogenesis of the embryonic palate and other organs. The present report describes our initial investigation of the Fraser-Juriloff paradigm, which proposes that susceptibility to malformation results from genetically determined differences in normal developmental patterns. Specifically, we evaluated the relationship between Igf2r gene expression, transforming growth factor-beta (TGF-beta) activation, and cdk4 gene expression. By using in situ hybridization, RNase protection assays, indirect immunofluorescence, Western blots, and bioassays, we show 1) the presence of insulin-like growth factor II (IGF-II), IGF-II receptor (IGF-IIR), IGF-IR, TGF-beta, plasminogen, plasminogen activators [urokinase plasminogen activator (uPA) and tissue plasminogen activator (tPA)], and Cdk4 in developing palates; 2) on embryonic day 14 (E14), which is a critical day for palatal growth, B10.A embryos have 82% greater IGF-IIR mRNA than B10; 3) on E14, B10.A embryonic palates have a 57% greater level of active TGF-beta2 than B10, although the total TGF-beta2 is nearly identical; and 4) on E14, B10 embryonic palates have a 52% greater...Continue Reading

References

Aug 1, 1975·The Journal of Pediatrics·J W Hanson, D W Smith
Jan 1, 1979·Differentiation; Research in Biological Diversity·D S Salomon, R M Pratt
Jan 1, 1992·American Journal of Medical Genetics·M Melnick
Jan 1, 1992·Breast Cancer Research and Treatment·F Vignon, H Rochefort
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P A Dennis, D B Rifkin
Jan 1, 1990·Immunogenetics·V VincekJ Klein
Oct 1, 1988·Toxicology and Applied Pharmacology·D K HansenT F Grafton
Feb 1, 1988·American Journal of Medical Genetics·M J KhouryT H Beaty
Sep 1, 1988·Toxicology·L Johansson, C E Pellicciari
Jan 1, 1986·The Biochemical Journal·F J BallardJ C Wallace
Dec 1, 1972·The Journal of Obstetrics and Gynaecology of the British Commonwealth·J Andrews, J M McGarry
Jul 1, 1981·Clinical Genetics·E D ShieldsP Fogh-Andersen
Dec 8, 1995·Science·D P Barlow
Jan 1, 1995·American Journal of Respiratory Cell and Molecular Biology·B M Moats-StaatsA D Stiles
Apr 1, 1995·Endocrine Reviews·D LeRoithC T Roberts
Jan 1, 1995·Teratology·F C Fraser, A Sajoo
Dec 1, 1994·Trends in Biochemical Sciences·R Derynck
Feb 1, 1994·The Journal of Clinical Investigation·M H Barcellos-HoffJ A Weatherbee
Dec 15, 1993·Biochemical and Biophysical Research Communications·Y XuC Polychronakos
Oct 1, 1995·Human Molecular Genetics·O W SmrzkaD P Barlow
Feb 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S Ghosh, P R Brauer
Nov 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M Agrawal, P R Brauer

❮ Previous
Next ❯

Citations

Sep 24, 2013·Genetic Testing and Molecular Biomarkers·Mingrui TangDi Wang
Jun 8, 2013·ILAR Journal·Ratnam S SeelanRobert M Greene
Apr 1, 2006·Cytogenetic and Genome Research·T H VuA R Hoffman
Jul 8, 2015·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Norliana GhazaliSaidi Jaafar
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M Melnick, T Jaskoll
Jul 6, 2000·Molecular Cell·J K KillianR L Jirtle
Jan 10, 2009·Journal of Dental Research·L MengJ W Von den Hoff
Dec 15, 2007·Virchows Archiv : an International Journal of Pathology·Silvia Vanessa LourençoFernando Augusto Soares
Jul 1, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayRobert M Greene
Aug 7, 2019·Birth Defects Research·Ratnam S SeelanRobert M Greene
Sep 3, 2020·Tissue Engineering. Part B, Reviews·Jeremie D OliverRena N D'Souza
Sep 5, 2019·International Journal of Molecular Sciences·Jia LiuJuan Du

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.