Insulin-like growth factor II regulation of gene expression in rat and human hepatomas

Journal of Cellular Physiology
I ZvibelL M Reid

Abstract

Insulin-like growth factor II (IGF II) regulated tissue-specific gene expression in hepatoma cell lines, but had no effect on expression of tissue-specific genes in primary cultures of E14 and newborn rat liver cells depleted of erythroid cells. No change was observed in these primary cultures with respect to alpha-fetoprotein (alpha-FP), albumin, cytokeratin 19 (CK19), gamma-glutamyltranspeptidase (GGT), and IGF II receptors. Two well-differentiated hepatoma, HepG2 and FTO-2B, and a poorly differentiated hepatoma, H4AzC2, did not show increased proliferation in the presence of IGF II, yet showed gene expression changes in response to IGF II. In HepG2 cells, IGF II increased albumin mRNA levels and resulted in a shift from clusters of cells positive to 100% of the cells expressing immunohistochemically detectable albumin. The transcription factor HNF-3 beta mRNA and protein levels of the bile duct markers, CK19 and GGT, were also increased in the presence of IGF II. Other genes tested were not affected, including alpha-1-antitrypsin, and two liver-specific transcription factors, HNF-4 and HNF-3 alpha. In FTO-2B cells, IGF II increased the expression of albumin, CK19, and GGT, without accompanying changes in albumin and GGT mRNA...Continue Reading

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Citations

Mar 24, 2009·The Journal of Reproduction and Development·Takeshi TeramuraYoshihiko Osoi
Aug 7, 2012·European Journal of Internal Medicine·C MoraceA Spadaro
Mar 15, 2001·European Journal of Gastroenterology & Hepatology·T NagaiJ Takahara
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·I ZvibelM Papa

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