Abstract
Insulin receptor substrate 1 (IRS-1) is the principle cellular substrate for insulin and insulin-like growth factor I (IGF-I) receptor signaling. After phosphorylation of tyrosine residues within the YMXM or YXXM motifs, IRS-1 associates with phosphatidylinositol-3 kinase (PI3K). This signaling pathway and the presence of an IRS-1-like molecule have been demonstrated in IRS-1-transfected and in nontransfected hematopoietic cell lines, respectively. IGF-I has been implicated in lymphocyte development and function, and recently, we showed that functional type-I IGF receptors are present on human thymocytes and peripheral T cells. In this study, we addressed IGF-I signal transduction in nontransformed, freshly isolated, human thymocytes, as well as in blood T cells. Using Western blot analysis, we found that IGF-I induced phosphorylation of a 160-180-kD protein (pp170) in human thymocytes and that phosphorylated pp170 becomes associated with PI3K and is recognized by anti-IRS-1. In blood T cells, this immunoreactive IRS-1 (irIRS-1) is less abundantly expressed than in thymocytes, as assessed with immunoblotting. As a consequence, phosphorylated pp170 was not or hardly detectable after stimulation with IGF-I, and irIRS-1 was not de...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S E ShoelsonM F White
Jan 1, 1992·Journal of Cellular Biochemistry·R A RothK Kovacina
May 1, 1992·Journal of Neuroimmunology·R KooijmanB J Zegers
Nov 1, 1992·Endocrinology·R KooijmanB J Zegers
Dec 1, 1992·Brain, Behavior, and Immunity·K W KelleyY M Li
Jul 4, 1991·Nature·X J SunM F White
Jan 30, 1990·Biochemical and Biophysical Research Communications·R N MargolisA L Hubbard
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C H JuneL E Samelson
Nov 14, 1985·Nature·M F WhiteC R Kahn
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N PerrottiS I Taylor
Sep 1, 1988·The Journal of Clinical Investigation·V F TapsonJ S Berman
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1983·Acta Endocrinologica·R M SchimpffP Chatelain
Mar 7, 1994·FEBS Letters·S UgiA Kashiwagi
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M WangS A Aaronson
May 14, 1993·Biochemical and Biophysical Research Communications·K S Kovacina, R A Roth
Apr 1, 1995·European Journal of Immunology·R K KooijmanB J Zegers
Aug 1, 1993·The Journal of Clinical Investigation·R ClarkP Jardieu
Sep 17, 1993·Science·L M WangJ H Pierce
Apr 1, 1993·Endocrinology·M G MyersM F White
Sep 1, 1993·The Journal of Clinical Investigation·H K Dadi, C M Roifman
Apr 15, 1993·Cellular Immunology·A Bajpai, Z Brahmi
Citations
Feb 18, 1999·Journal of Cellular Biochemistry·A M MartelliP Narducci
Oct 14, 2004·Journal of Cancer Research and Clinical Oncology·Maria José OliveiraAncy Leroy
Dec 20, 2011·Age·Christine MeyerIlhem Messaoudi
May 15, 2010·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Iker López-CaldereroRocío García-Carbonero
Nov 2, 2013·Cellular Immunology·Douglas A Weigent
Mar 1, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·R W JohnsonK W Kelley
Sep 28, 2007·Nature Clinical Practice. Oncology·Yungan TaoEric Deutsch
Jun 18, 1998·Annals of the New York Academy of Sciences·K W KelleyR Dantzer
Dec 25, 2015·Nature Reviews. Rheumatology·Andras Perl
May 9, 2000·Biochemical Pharmacology·T D StephensB J Fillmore
Aug 18, 2012·Cytokine·Alexander PuzikChristoph Härtel
Jan 11, 2011·Cancer Cell·Sarat ChandarlapatyNeal Rosen
Oct 12, 2014·Biochemical and Biophysical Research Communications·Chunfeng HouYanliang Lin
Jul 20, 2001·Medical Hypotheses·M F McCarty
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Morteza MotallebnezhadMehdi Yousefi
Aug 26, 2017·Arthritis & Rheumatology·Andras Perl
Apr 1, 1997·Endocrine Reviews·R Clark
Jun 1, 1997·Endocrinology·A G ScrimgeourD LeRoith
Jun 17, 2000·Endocrine Reviews·K Dorshkind, N D Horseman
Aug 22, 2007·Journal of Animal Science·J A Carroll
Jul 6, 2019·Journal of Diabetes and Metabolic Disorders·Ariel WilliamsK Sean Kimbro
May 4, 2011·World Journal of Diabetes·Cheng-Ming Wang, Bernhard Kaltenboeck
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·D H SchacherK W Kelley