Insulin-Like Growth Factors Are Expressed in the Taste System, but Do Not Maintain Adult Taste Buds

PloS One
Bradley T BiggsRobin F Krimm

Abstract

Growth factors regulate cell growth and differentiation in many tissues. In the taste system, as yet unknown growth factors are produced by neurons to maintain taste buds. A number of growth factor receptors are expressed at greater levels in taste buds than in the surrounding epithelium and may be receptors for candidate factors involved in taste bud maintenance. We determined that the ligands of eight of these receptors were expressed in the E14.5 geniculate ganglion and that four of these ligands were expressed in the adult geniculate ganglion. Of these, the insulin-like growth factors (IGF1, IGF2) were expressed in the ganglion and their receptor, insulin-like growth factor receptor 1 (IGF1R), were expressed at the highest levels in taste buds. To determine whether IGF1R regulates taste bud number or structure, we conditionally eliminated IGF1R from the lingual epithelium of mice using the keratin 14 (K14) promoter (K14-Cre::Igf1rlox/lox). While K14-Cre::Igf1rlox/lox mice had significantly fewer taste buds at P30 compared with control mice (Igf1rlox/lox), this difference was not observed by P80. IGF1R removal did not affect taste bud size or cell number, and the number of phospholipase C β2- (PLCβ2) and carbonic anhydrase 4...Continue Reading

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Citations

Sep 29, 2017·Complementary Medicine Research·Shipeng SunBaojin Hua
May 28, 2019·Chemical Senses·Sean M CrossonSergei Zolotukhin
Jul 31, 2018·Endocrinology·Máire E DoyleJosephine M Egan

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Methods Mentioned

BETA
PCR
laser capture microdissection

Key Resources (RRID) Mentioned

AB_2070332
AB_632197

Software Mentioned

Beacon Designer
Neurolucida

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