PMID: 2105494Feb 1, 1990Paper

Insulin, oxytocin, and vasopressin stimulate protein kinase C activity in adipocyte plasma membranes

Proceedings of the National Academy of Sciences of the United States of America
J J EganC Londos

Abstract

Incubation of isolated rat adipocytes with insulin, vasopressin, or oxytocin increased plasma membrane-bound protein kinase C (PKC) activity by 100-400%. PKC activity was assayed by a procedure that is virtually background-free, thus permitting assay of protein kinase activity in highly diluted samples of solubilized membranes. Hormone-dependent increases in PKC activity were limited to plasma membranes. Stimulation of the kinase was half-maximal with 70 pM insulin, and the hormone effect was rapid. Oxytocin and vasopressin produced effects on PKC similar to insulin, but the magnitude of the vasopressin stimulation exhibited seasonal variations. Treatment of cells with phorbol 12-myristate 13-acetate (PMA) resulted in a loss of PKC activity from the cytosol and a gain in plasma membrane activity, indicative of translocation of the enzyme. With activity measurements it was not possible to determine if insulin stimulated a translocation of the kinase. However, Western blot analysis of plasma membranes with polyclonal antibodies directed against PKC suggest that at least some of the insulin-stimulated PKC activity resulted from enzyme translocation.

References

Jun 1, 1979·Journal of Cellular Physiology·L S Lee, I B Weinstein
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R WhiteT Ishizaka
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K T YuM P Czech
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Smal, P De Meyts
Oct 1, 1986·Journal of Cellular Physiology·A Rodriguez-Pena, E Rozengurt
Aug 17, 1987·FEBS Letters·S I WalaasO Walaas
Oct 14, 1988·Biochemical and Biophysical Research Communications·B DrazninN A Sherman
May 2, 1985·European Journal of Biochemistry·G SkoglundM Ingelman-Sundberg
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M M CivanT G O'Brien
Jul 15, 1988·Biochemical and Biophysical Research Communications·P H AndersenH Juhl
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 30, 1987·Biochemical and Biophysical Research Communications·J Smal, P De Meyts
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SommercornE G Krebs
Jan 18, 1988·Biochimica Et Biophysica Acta·S M Hernández-Sotomayor, J A García-Sáinz
Mar 28, 1986·Biochemical and Biophysical Research Communications·B P GlynnL A Witters
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D TabariniO M Rosen
Jun 1, 1984·Molecular and Cellular Endocrinology·B Koepfer-Hobelsberger, O H Wieland

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Citations

Jul 13, 1994·Molecular and Cellular Biochemistry·S L Macaulay, R G Larkins
Sep 1, 1995·Diabetes Research and Clinical Practice·T IshizukaY Nozawa
Aug 1, 1996·Diabetes Research and Clinical Practice·T IshizukaY Okano
Jan 1, 1991·Cellular Signalling·G Grunberger
Mar 1, 1992·Cellular Signalling·R V FareseD R Cooper
Oct 1, 1993·Baillière's Clinical Endocrinology and Metabolism·W T Garvey, M J Birnbaum
Aug 26, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Andreas SchäfflerChrista Buechler
Dec 29, 1995·Annals of the New York Academy of Sciences·S J LolaitM J Brownstein
May 16, 2014·International Journal of Molecular Sciences·Rajesh L ThangapazhamJon Meyerle
Oct 15, 1992·Biochemical and Biophysical Research Communications·D R CooperR V Farese
Jun 29, 1990·Biochemical and Biophysical Research Communications·T IshizukaR V Farese
Dec 1, 1992·Archives of Biochemistry and Biophysics·H A SingerH Benscoter
Mar 16, 1992·Biochemical and Biophysical Research Communications·T IshizukaR V Farese
May 1, 2013·Peptides·Valéria Ernestânia ChavesMárcia Nascimento Brito
Feb 26, 1991·Biochemistry·R M EpandM T Debanne
Mar 30, 2004·Journal of Cellular Biochemistry·Paramita M GhoshJeffrey I Kreisberg
Sep 27, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·C DingF Magkos
Mar 6, 2003·Physiological Genomics·Stephanie R WesolowskiDaniel Pomp
May 5, 1995·The Journal of Biological Chemistry·B FèveA D Strosberg
Nov 17, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T Dylan OlverJaume Padilla

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