Insulin receptor content in tissues of normal and diabetic rats measured by radioimmunoassay

Journal of Endocrinological Investigation
V PezzinoV Papa

Abstract

Insulin receptor (IR) content in different tissues has been quantitatively evaluated by means of steady state binding studies with radiolabeled insulin. The information provided by this approach, however, does not give a direct measurement of the receptor protein. Rather, it depends on the binding function of the IR, evaluated on the basis of curvilinear plots derived by Scatchard analysis of the experimental data. In the present report we employed a sensitive and specific radioimmunoassay (RIA) that allows a direct measurement of IR in solubilized cells or tissues. By this method we studied: a) IR distribution in several tissues of the rat, the animal model most frequently used in studies of insulin action; b) IR regulation in streptozotocin-treated, diabetic insulin deficient rats. Tissues from male Wistar rats (11 controls and 6 streptozotocin-treated diabetic animals) were homogenized, solubilized with Triton X-100 in the presence of protease inhibitors and stored at -80 C. IR content in the solubilized material was then measured by RIA. IR were detectable in all 11 tissues tested. Liver, kidney and brain neocortex had the highest IR content. (24.7 +/- 1.0, 20.5 +/- 1.1, 25.9 +/- 1.6 ng/mg protein, m +/- SE, respectively). ...Continue Reading

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Citations

Jul 2, 1999·Journal of Sleep Research·J L ValatxD Bucchini
Mar 12, 2002·Clinical & Experimental Ophthalmology·Andrew D GosbellPaula Jablonski
Jul 19, 2012·Bulletin of Experimental Biology and Medicine·O V ChistyakovaA O Shpakov
Dec 4, 2019·Frontiers in Aging Neuroscience·Domenico SergiMaria-Grazia Martinoli

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