PMID: 6171829Jan 1, 1981Paper

Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes

Progress in Clinical and Biological Research
M D LaneP R Clements

Abstract

3T3-L1 "preadipocytes" can be induced to differentiate in culture into cells having the morphological and biochemical characteristics of adipocytes. The binding of 125I-insulin to the cell-surface of differentiated and undifferentiated 3T3-L1 cells and nondifferentiating 3T3-C2 cells was compared. In the absence of agents which induce adipocyte conversion, ie, insulin or insulin plus methylisobutylxanthine (MIX) and dexamethasone (DEX), 3T3-L1 cells fail to express the adipocyte phenotype and maintain a constant number of insulin binding sites. Induction of adipocyte conversion with 3T3-L1 cells in the presence of insulin causes apparent down-regulation of insulin receptors followed by a 12--15-fold increase in receptor number which parallels differentiation. Approximately 170,000 insulin binding sites per cell are expressed when greater than 75% of the cells have differentiated. The rise of insulin receptor level is differentiation-dependent. 3T3-C2 cells, which do not differentiate in the presence of insulin or insulin plus MIX and DEX, exhibit only insulin-induced down-regulation of insulin receptors. The increase of insulin receptor level in 3T3-L1 cells in receptor-specific since the levels of epidermal growth factor recep...Continue Reading

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