Insulin reduces apoptosis and increases DNA synthesis and cell size via distinct signalling pathways in Drosophila Kc cells

Cell Proliferation
G BikopoulosA J Hilliker

Abstract

During development of Drosophila, cell proliferation and size are known to be regulated by insulin. Here we use Drosophila Kc cells to examine the molecular basis for the control of cell growth by insulin. Growing cells in the presence of insulin increased cell number above control levels at 16, 24, 48 and 72 h. We have demonstrated a novel anti-apoptotic effect of insulin (approximately 50%) in these cells, measured by caspase 3-like activity, which contributed to the increase in cell number. The anti-apoptotic effect was observed both in control cells and those in which apoptosis was induced by ultraviolet irradiation. An approximately 2-fold stimulation of bromodeoxyuridine incorporation demonstrated that insulin also increased Kc cell proliferation by stimulating new DNA synthesis. The ability of insulin to increase cell number, stimulate bromodeoxyuridine incorporation and reduce caspase 3-like activity was prevented by PD98059, which inhibits activation of the Drosophila extracellular signal regulated kinase (DERK) pathway, and was unaffected by wortmannin, an inhibitor of Drosophila phosphatidylinositol 3-kinase (DPI3K). Insulin also increased cell size approximately 2-fold and this was prevented by wortmannin and rapamy...Continue Reading

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·K L WatsonR L Erikson
Feb 7, 1998·The Journal of Biological Chemistry·S J HanW J Lee
Feb 28, 1998·The Journal of Biological Chemistry·F BertrandG Cherqui
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Feb 13, 1999·Cell·D L Vaux, S J Korsmeyer
Mar 24, 1999·Trends in Biochemical Sciences·S Kumar, P A Colussi
Dec 1, 1999·Cell Death and Differentiation·D W Nicholson
Dec 10, 1999·Nature Cell Biology·J VerduM J Birnbaum
Dec 10, 1999·Nature Cell Biology·B A Edgar
May 10, 2000·The Journal of Biological Chemistry·R M PoltiloveS I Taylor
May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Bangs, K White
Jan 4, 2001·Cell Death and Differentiation·S Kumar, J Doumanis
Apr 13, 2001·Current Biology : CB·S J Leevers
Nov 8, 2001·Current Opinion in Cell Biology·N TaponI K Hariharan
Jan 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Laura A Johnston, Peter Gallant
Feb 9, 2002·The Journal of Biological Chemistry·Hyung-Bae KwonKang-Yell Choi
Feb 28, 2002·Nature Cell Biology·Thomas RadimerskiGeorge Thomas
Apr 12, 2002·Trends in Endocrinology and Metabolism : TEM·Robert S Garofalo
Jun 20, 2002·Journal of Immunological Methods·Helena Richardson, Sharad Kumar
Feb 1, 2003·Trends in Cell Biology·Sean Oldham, Ernst Hafen
Mar 13, 2003·Kidney International·Takao MasakiDavid J Nikolic-Paterson

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis