Insulin-regulated protein palmitoylation impacts endothelial cell function

Arteriosclerosis, Thrombosis, and Vascular Biology
Xiaochao WeiClay F Semenkovich

Abstract

Defects in insulin signaling are associated with abnormal endothelial cell function, which occurs commonly in cardiovascular disease. Targets of insulin signaling in endothelial cells are incompletely understood. Protein S-palmitoylation, the reversible modification of proteins by the lipid palmitate, is a post-translational process relevant to cell signaling, but little is known about the role of insulin in protein palmitoylation. To test the hypothesis that insulin alters protein palmitoylation in endothelial cells, we combined acyl-biotin exchange chemistry with stable isotope labeling by amino acids in cell culture to perform quantitative proteomic profiling of human endothelial cells. We identified ≈380 putative palmitoylated proteins, of which >200 were not known to be palmitoylated; ≈10% of the putative palmitoylated proteins were induced or suppressed by insulin. Of those potentially affected by insulin, <10 have been implicated in vascular function. For one, platelet-activating factor acetylhydrolase IB subunit gamma (PAFAH1b3; not previously known to be palmitoylated), we confirmed that insulin stimulated palmitoylation without affecting PAFAH1b3 protein abundance. Chemical inhibition of palmitoylation prevented insul...Continue Reading

References

Apr 23, 1992·Biochimica Et Biophysica Acta·R A ColemanE S Bardes
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Piero O BonettiAmir Lerman
Apr 16, 2003·Biochemistry·Maurine E Linder, Robert J Deschenes
May 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wei YanGary D Clark
Dec 4, 2003·EMBO Reports·W Scott ArgravesWilliam M Gallagher
Jun 6, 2006·Cell·Amy F RothNicholas G Davis
Nov 2, 2006·Science's STKE : Signal Transduction Knowledge Environment·Marilyn D Resh
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Maurine E Linder, Robert J Deschenes
Jun 23, 2007·Nature Protocols·Junmei WanNicholas G Davis
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Matthias P Wymann, Roger Schneiter
Jun 10, 2008·The New England Journal of Medicine·UNKNOWN Action to Control Cardiovascular Risk in Diabetes Study GroupWilliam T Friedewald
Jul 25, 2008·Nature·Steven J Bensinger, Peter Tontonoz
Sep 9, 2008·Endocrinology and Metabolism Clinics of North America·Babak RazaniClay F Semenkovich
Dec 19, 2008·Nature·Rujun KangAlaa El-Husseini
Jan 13, 2009·Nature Methods·Brent R Martin, Benjamin F Cravatt
Apr 9, 2009·Cancer Research·Shou-Ching ShihHarold F Dvorak
Jul 16, 2009·Journal of Cellular and Molecular Medicine·Yan LiuManuela Martins-Green
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Barth D Grant, Julie G Donaldson
Oct 6, 2009·Molecular & Cellular Proteomics : MCP·Wei YangMichael R Freeman
Nov 6, 2009·Molecular Cancer Research : MCR·Bin HuMariano S Viapiano
Mar 17, 2010·The New England Journal of Medicine·William C CushmanFaramarz Ismail-Beigi
Mar 17, 2010·The New England Journal of Medicine·Henry N GinsbergRobert P Byington
Jun 19, 2010·Nature Chemical Biology·Rami N Hannoush, Jinglucy Sun
Jul 14, 2010·The Journal of Cell Biology·Marie E BechlerWilliam J Brown
Nov 26, 2010·Cellular Immunology·Hyouna YooMyung Kim
Nov 26, 2010·The Journal of Biological Chemistry·Xiaochao WeiClay F Semenkovich
May 20, 2011·Molecular Biology of the Cell·Marie E BechlerWilliam J Brown
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Dec 24, 2011·Frontiers in Physiology·Andrea M FoskettMariappan Muthuchamy
Feb 22, 2012·Cell Host & Microbe·Xiaochao WeiClay F Semenkovich
Feb 22, 2012·Trends in Molecular Medicine·Marilyn D Resh
Apr 14, 2012·Nature Reviews. Molecular Cell Biology·Victor W HsuJian Li
Aug 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Christian Rask-Madsen, C Ronald Kahn

❮ Previous
Next ❯

Citations

Jun 27, 2015·Biochimica Et Biophysica Acta·Marc Yeste-VelascoYong-Jie Lu
Jul 6, 2016·Nature Chemical Biology·Baoen ChenXu Wu
Jun 8, 2017·Nature Communications·Joshua M BoucherVictoria L Bautch
Jul 13, 2018·Critical Reviews in Biochemistry and Molecular Biology·María-Eugenia Zaballa, F Gisou van der Goot
Aug 28, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Sara N Vallerie, Karin E Bornfeldt
Sep 5, 2015·F1000Research·Mathieu BlancFrançoise Gisou van der Goot
Feb 12, 2019·Annual Review of Physiology·Xuri LiPeter Carmeliet
Nov 24, 2017·Physiological Reviews·Guy EelenPeter Carmeliet
Jul 18, 2019·Molecular & Cellular Proteomics : MCP·Monika Zareba-KoziolJakub Wlodarczyk
Mar 11, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mohamed A ZayedClay F Semenkovich
Aug 30, 2017·The Journal of Biological Chemistry·Kasturi RoyEthan P Marin
Feb 6, 2017·Journal of Neuroscience Research·Sabrina M Holland, Gareth M Thomas
Oct 16, 2018·Frontiers in Molecular Neuroscience·Alexandre DumoulinFritz G Rathjen
Dec 6, 2018·International Journal of Molecular Sciences·Matteo SpinelliClaudio Grassi
Jul 12, 2020·Communications Biology·Eliot MorrisonChristian Freund
Jan 7, 2017·The Journal of Biological Chemistry·Keyong DuBernhard Luscher
Oct 1, 2019·Molecular & Cellular Proteomics : MCP·Monika Zareba-KoziolJakub Wlodarczyk
Oct 24, 2020·Journal of Cellular Physiology·Jiayu JinDan Meng
Apr 1, 2021·Open Biology·Luke H ChamberlainGwyn W Gould
May 27, 2021·Endocrinology·Andrew M N WalkerRichard M Cubbon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

The Journal of Cell Biology
Jennifer Greaves, Luke H Chamberlain
Biochemical Society Transactions
Mathieu BlancF Gisou Van Der Goot
The Journal of Biological Chemistry
Michael J Shipston
The FEBS Journal
Sanja BlaskovicF Gisou Van Der Goot
© 2021 Meta ULC. All rights reserved