PMID: 7529202Jan 1, 1995Paper

Insulin secretagogues, but not glucose, stimulate an increase in [Ca2+]i in the fetal rat beta-cell

Diabetes
A J WeinhausB E Tuch

Abstract

Fetal pancreatic islets release insulin poorly in response to glucose; however, the cellular mechanism for this is controversial. By using fura 2 to measure changes in the cytoplasmic free Ca2+ concentration ([Ca2+]i) in beta-cells, we have examined islets from fetal, neonatal, and adult rats to determine the ability of glucose and other secretagogues to cause an increase in [Ca2+]i. The effects of glucose (20 mmol/l), glyceraldehyde (20 mmol/l), leucine (20 mmol/l), arginine (20 mmol/l), and the channel effectors glipizide (50 mumol/l), BAY K8644 (2 mumol/l), diazoxide (300 mumol/l), and verapamil (20 mumol/l) on changes in [Ca2+]i were studied. In both the fetal and the mature islet, glyceraldehyde, leucine, arginine, glipizide, and BAY K8644 caused an increase in [Ca2+]i. In mature islets, glucose also increased [Ca2+]i; however, in the fetal islet, glucose had no effect on [Ca2+]i. The stimulus-induced increases in [Ca2+]i in fetal and adult islets were both significantly inhibited by the addition of either diazoxide or verapamil. Similar results were obtained when insulin secretion was measured. Our data show that various secretagogues are able to stimulate fetal islets and cause an increase in [Ca2+]i. Glucose, however, f...Continue Reading

Citations

Feb 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F MirallesR Scharfmann
Jun 14, 2002·Nursing Research·Linda Ciofu BaumannRoberta Hoebeke
Nov 6, 2008·Acta Physiologica·M Rupnik
Jul 17, 1998·The Journal of Clinical Investigation·T YamaokaM Itakura
Sep 21, 2005·The International Journal of Biochemistry & Cell Biology·Po Man SuenPo Sing Leung
Aug 18, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Ayman HyderJürgen Schrezenmeir
Sep 15, 2016·International Journal of Molecular Sciences·Milou Groot NibbelinkAart Van Apeldoorn
Mar 21, 2018·Frontiers in Endocrinology·Neivys García-DelgadoMarcia Hiriart
Mar 1, 1997·The Journal of Clinical Endocrinology and Metabolism·J Tu, B E Tuch
Feb 11, 2005·The Journal of Biological Chemistry·Hiroshi IwakuraKazuwa Nakao

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