Insulin secretion and islet lysosomal enzyme activities in the mouse. Effects of suramin

International Journal of Pancreatology : Official Journal of the International Association of Pancreatology
G SkoglundI Lundquist

Abstract

To study the functional role of the lysosomes in islet physiology, we have in the present study investigated influences on insulin secretion induced by a primary inhibition of islet lysosomal enzyme activities by the use of the lysosomotropic drug suramin. First, we demonstrated that the activities of the three lysosomal enzymes, acid amyloglucosidase, acid alpha-glucosidase, and N-acetyl-beta-D-glucosaminidase, were inhibited in mouse islet homogenates upon direct addition of suramin (p less than 0.001). Thereafter, we studied the influences of suramin on islet lysosomal enzyme activities at 24 h after an administration of suramin to mice. We thereby found that the islet lysosomal enzyme activities were not significantly altered compared to controls. However, after incubation of the islets at 3.3 mM glucose, the activities of acid amyloglucosidase and acid alpha-glucosidase were increased (p less than 0.05). The activity of N-acetyl-beta-D-glucosaminidase was, however, not affected. Hence, the direct inhibitory action on islet lysosomal enzyme activities by suramin seems to be counteracted in vivo. Concurrently with the increased enzyme activities in incubated islets, glucose-induced insulin secretion was significantly potenti...Continue Reading

References

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