Insulin stimulates lipoprotein lipase activity and synthesis in adipocytes from septic rats

The Journal of Surgical Research
E F RosatoS Lanza-Jacoby

Abstract

Gram-negative sepsis suppresses lipoprotein lipase (LPL) activity in adipose tissue which contributes, in part, to the altered clearance of triglycerides. The suppression in LPL activity occurs when plasma insulin concentrations are elevated and insulin-stimulated glucose utilization is impaired. This study was planned to evaluate whether the presence of insulin resistance was responsible for the decrease in adipose LPL activity. Adipocytes were isolated from epididymal fat pads 24 h after inducing sepsis in male Lewis rats by intravenous injection of 4 x 10(8) colonies of live Escherichia coli/100 g body wt. The decrease in heparin-releasable (HR) LPL activity in adipocytes from the septic rats was evident at the time of isolation and maintained in a 20-h culture. After overnight incubation with insulin (10(-8) M), HR LPL activity was stimulated to a greater extent in adipocytes from septic rats (298%) than in adipocytes from control rats (88%). The insulin stimulation of LPL activity during sepsis could not be attributed to insulin-like growth factor-I (IGF-I) as adipocytes from septic rats appeared to be IGF-I resistant. Insulin-treatment (10(-8) M) increased LPL synthesis 99% in adipocytes from control rats and 136% in adip...Continue Reading

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Citations

Jun 16, 2009·Annals of Biomedical Engineering·Katarina JelicMorten Colding-Jørgensen
Nov 23, 2006·Infection and Immunity·Mahalia S DesruisseauxPhilipp E Scherer
Feb 7, 2006·General and Comparative Endocrinology·Amaya AlbalatIsabel Navarro
Jan 3, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Karine GontierStéphane Davail
Dec 6, 2003·Journal of Leukocyte Biology·Soren Kaeseler AndersenElse Tønnesen
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F PicardY Deshaies
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuchen MaJoseph L Messina
Jun 3, 2021·Journal of Clinical Medicine·Weronika Wasyluk, Agnieszka Zwolak

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