PMID: 3033636May 1, 1987Paper

Insulin stimulates phosphorylation of a 120-kDa glycoprotein substrate (pp120) for the receptor-associated protein kinase in intact H-35 hepatoma cells

Proceedings of the National Academy of Sciences of the United States of America
N PerrottiS I Taylor

Abstract

The insulin receptor possesses protein kinase activity, which may play a role in mediating insulin action. Recently, we have identified a glycoprotein (pp120) in rat liver plasma membranes that is phosphorylated by the solubilized insulin receptor in a cell-free system. We now report that insulin stimulates phosphorylation of pp120 in intact H-35 cells. H-35 cells were preloaded with [32P]orthophosphate to label the intracellular ATP pool. Insulin caused a 10-fold increase in the phosphorylation of its receptor and a 2-fold increase in phosphorylation of pp120 (P less than 0.001). The time course of insulin's stimulation of pp120 closely paralleled that of insulin receptor phosphorylation over the time period investigated (15-45 min). This effect had the specificity corresponding to the insulin receptor. Epidermal growth factor was inactive, and insulin-like growth factor I had approximately equal to 1% the potency of insulin in this regard. Insulin increased 32P incorporation into pp120 in a linkage that was stable to alkaline hydrolysis, as would be expected for tyrosine-specific phosphorylation. Direct phosphoamino acid analysis confirmed that insulin increased 32P incorporation into phosphotyrosine residues in pp120.

References

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Citations

Jan 1, 1991·Journal of Clinical Laboratory Analysis·J A ThompsonW Zimmermann
Sep 30, 1988·Biochemical and Biophysical Research Communications·K MomomuraM Kasuga
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R N MargolisA L Hubbard
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A SoosK Siddle
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M ChuangC R Kahn
Nov 26, 1990·European Journal of Biochemistry·Y Biener, Y Zick
Jan 30, 1990·Biochemical and Biophysical Research Communications·R N MargolisA L Hubbard
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·Y Zick
Sep 3, 2004·American Journal of Physiology. Cell Physiology·Miranda MennitiNicola Perrotti
Sep 3, 1990·FEBS Letters·A BianchiL A Witters
Apr 11, 2019·Physiology·Sonia M Najjar, Germán Perdomo
Sep 6, 1996·The Journal of Biological Chemistry·M J SaadL A Velloso
Mar 14, 2014·Reviews in the Neurosciences·Dema NajemWandong Zhang
Oct 26, 1999·The Journal of Biological Chemistry·B H QuD LeRoith
Oct 14, 2018·International Journal of Molecular Sciences·Andrea Kristina HorstGisa Tiegs
Dec 10, 2000·The Journal of Biological Chemistry·N PerrottiS I Taylor
Nov 14, 1997·The American Journal of Physiology·S Li CalziS M Najjar
Jun 27, 1998·Biochemical and Biophysical Research Communications·S M Najjar

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