Insulin up-regulates epithelial sodium channel in LPS-induced acute lung injury model in rats by SGK1 activation

Injury
Tao ZhuDao-Xin Wang

Abstract

Activity of the epithelial sodium channels (ENaCs) in the lung tissue plays a critical role on sodium/fluid homeostasis and the lung fluid clearance. The serum- and glucocorticoid-inducible kinase-1 (SGK1), one of the critical regulation proteins of ENaC, is activated by insulin and growth factors possibly through 3-phosphoinositide-dependent kinase PDK1 or/and phosphatidylinositol 3-kinase (PI3K). However, it is uncertain whether insulin shows its stimulatory action on ENaC by activation of SGK1 in lipopolysaccharide (LPS)-induced acute lung injury (ALI) condition. In our study, Wistar rats were injected with LPS to induce ALI. Evans blue dye albumin (EBA) concentration was used to measure pulmonary oedema. For detecting the ratio of phospho-SGK1/SGK1 and α-ENaC protein, Western blot was performed. Real-time polymerase chain reaction (RT-PCR) was used to assess α-ENaC messenger RNA (mRNA). Immunohistochemistry was used to locate and quantitate α-ENaC expression. The EBA concentration was markedly increased by LPS alone but significantly reduced in rats that also received insulin injection. The ratio of phospho-SGK1/SGK1 was raised significantly in the insulin group and insulin+LPS group, compared with the control group and the...Continue Reading

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Citations

Apr 30, 2013·Critical Care : the Official Journal of the Critical Care Forum·Wei FanKoshi Makita
Dec 5, 2013·International Journal of Molecular Sciences·Tao ZhuHong Jin
Nov 14, 2013·BioMed Research International·Daniely Cornélio FavarinAlexandre de Paula Rogerio
Feb 4, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Qianchao WuHaihua Feng
Jun 27, 2014·International Immunopharmacology·Rui-Hua ZhangShu-Fei Tian
Oct 28, 2014·International Journal of Molecular Sciences·Min XiaoFu-Qiang Wen
Mar 20, 2013·Expert Opinion on Investigational Drugs·Florian Lang, Jakob Voelkl
Jun 15, 2013·The Journal of Surgical Research·Wei-ting ZhongDa-cheng Wang
Aug 16, 2016·International Journal of Molecular Sciences·Yiyun LouFan Jin
Aug 8, 2014·American Journal of Physiology. Renal Physiology·Tiffany L ThaiHui-Fang Bao
Sep 27, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Florian Lang, Ekaterina Shumilina

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