Insulinoma-induced hypoglycemic death in mice is prevented with beta cell-specific gene therapy

Annals of Surgery
Thomas A TironeF Charles Brunicardi

Abstract

Tumor-specific gene therapy can be achieved if a tumor-specific promoter can be identified. In this study the authors investigated the use of the rat insulin promoter (RIP) for insulinoma-specific expression of a reporter gene. Insulinoma-specific cytotoxicity using the suicide gene thymidine kinase (tk) was studied both in vitro and in vivo. RIPtk gene therapy, delivered by a nontoxic, noninflammatory liposomal delivery system, was used in an insulinoma ICR/SCID mouse model to prevent hypoglycemic death. Rat insulin promoter (0.502 kb) was ligated to the reporter gene lacZ and ligated to the tk gene. These two genes were transfected into a mouse insulinoma (NIT) cell line to ascertain insulinoma-specific expression and insulinoma-specific cytotoxicity in vitro. Reverse transcriptase-polymerase chain reaction and electrophoretic mobility-shift assays were performed on NIT-1 cell RNA and nuclear extract, respectively, to determine the transcription factors present and responsible for RIP activation in NIT-1 cells. A mouse insulinoma model was created with NIT-1 cells. These mice were treated with the RIPtk gene, and both blood sugars and animal viability were monitored. Only NIT-1 cells stained blue after X-gal staining or had d...Continue Reading

References

Mar 1, 1995·The Journal of Investigative Dermatology·B BonnekohD R Roop
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S H ChenS L Woo
Feb 24, 1997·Biochemical and Biophysical Research Communications·T TanakaM Omata
May 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M Sander, M S German
Jul 1, 1997·Nature Biotechnology·N S TempletonG N Pavlakis
Mar 11, 1998·The Australian and New Zealand Journal of Surgery·C A Proye
Nov 25, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·B Salvadori

❮ Previous
Next ❯

Citations

Feb 8, 2003·DNA and Cell Biology·Nancy Smyth Templeton
Mar 19, 2004·Pancreas·Satoshi AshizawaXiao-Ping Wang
Jun 2, 2012·Journal of Drug Delivery·Nancy Smyth Templeton, Neil Senzer
May 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shuyuan ChenPaul A Grayburn
Nov 22, 2008·World Journal of Surgery·Nancy Smyth Templeton
Dec 13, 2006·The Journal of Surgical Research·Shihe LiuF Charles Brunicardi
Jul 11, 2012·European Journal of Immunology·Jason MiskaZhibin Chen
May 18, 2004·The Journal of Surgical Research·Min LiWilliam E Fisher
Apr 30, 2003·Expert Opinion on Biological Therapy·Nancy Smyth Templeton
Dec 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Huanzhang LuNancy Smyth Templeton
Oct 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yufeng ZhangNancy Smyth Templeton
May 22, 2007·The Journal of Surgical Research·Shi-He LiuF Charles Brunicardi
Feb 12, 2005·World Journal of Surgery·Xiao-Ping WangF Charles Brunicardi
May 14, 2005·World Journal of Surgery·Kiichi TamadaF Charles Brunicardi
Feb 1, 2008·Acta Biochimica Et Biophysica Sinica·Yiqun ZhangXinyu Qin
Sep 17, 2009·The Journal of Physical Chemistry. B·Wen-Jay Lee, Shin-Pon Ju
Sep 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Wen-Jay Lee, Shin-Pon Ju

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.