Insulins in equine urine: qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse-racing

Rapid Communications in Mass Spectrometry : RCM
Tiia KuuranneMario Thevis

Abstract

Insulin is a peptide hormone consisting of two peptide chains (A- and B-chain) that are cross-linked by two disulfide bonds. To obtain improved pharmacokinetic onset of action profiles of insulin treatment in diabetic patients, recombinant long-, intermediate-, and rapid-acting insulin analogs are produced, in which the C-terminal end of the B-chain plays an especially important role.A review of the veterinary literature reveals the low prevalence of equine type I diabetes mellitus, which indicates that the therapeutic use of insulin in racing horses is unlikely. Although there is no unequivocal evidence of an overall performance-enhancing effect of insulin, in human sports the misuse of insulin preparations is reported among elite athletes. The desired effects of insulin include the increase of muscular glycogen prior to sports event or during the recovery phase, in addition to a chalonic action, which increases the muscle size by inhibiting protein breakdown. In the present study urinary insulin was detected in equine samples and differences between equine insulin, human insulin, as well as rapidly acting recombinant insulin variants were examined. The method was based on sample purification by solid-phase extraction (SPE) an...Continue Reading

References

Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·J Trethewey
Feb 21, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·H ShenR T Kennedy
Feb 24, 2001·Journal of Analytical Toxicology·S M DarbyM LeBeau
Jun 30, 2001·The Journal of Endocrinology·P H Sonksen
Aug 17, 2001·Rapid Communications in Mass Spectrometry : RCM·C FierensA P De Leenheer
Jan 30, 2002·Clinical Therapeutics·R K CampbellD Baker
May 24, 2003·Journal of Pharmaceutical and Biomedical Analysis·N F C VisserH Irth
Jan 8, 2004·Clinical Chemistry·William E Owen, William L Roberts
Jun 15, 2006·The Journal of Nutrition·Kibby H TreiberRaymond J Geor
Jun 15, 2006·The Journal of Nutrition·Patricia HarrisAnnette Longland
Jan 4, 2007·Rapid Communications in Mass Spectrometry : RCM·Mario ThevisWilhelm Schänzer

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Citations

Jan 16, 2013·Analytical and Bioanalytical Chemistry·Wai Him KwokTerence S M Wan
Aug 30, 2011·Methods : a Companion to Methods in Enzymology·Andreas ThomasMario Thevis
Jul 12, 2011·Domestic Animal Endocrinology·K D TinworthG K Noble
Oct 12, 2010·Journal of Chromatography. a·Maria LönnbergJan Carlsson
Jul 11, 2008·Journal of Separation Science·Roman TomásFrantisek Foret
Jun 24, 2011·Mass Spectrometry Reviews·Theodora KatsilaConstantin Tamvakopoulos
Sep 11, 2016·BMC Veterinary Research·T WarnkenK Feige
Apr 22, 2009·The Analyst·Eric EzanFrançois Becher
Oct 14, 2017·PloS One·Min YuanJohn M Asara
Mar 30, 2017·Drug Testing and Analysis·Tessa WilkinNatasha Hamilton
Feb 3, 2019·Cold Spring Harbor Protocols·James DeCaprio, Thomas O Kohl
Jan 14, 2021·Drug Testing and Analysis·Fernando MoreiraMaria de Lourdes Bastos
Jun 23, 2020·Journal of Proteomics·Elisabetta Chiaradia, Ingrid Miller

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