PMID: 2114401Jul 15, 1990Paper

Insulin's regulation of c-fos gene transcription in hepatoma cells.

The Journal of Biological Chemistry
J L Messina

Abstract

In H4IIE rat hepatoma cells insulin interacts with its receptors to induce DNA synthesis and promote cell division. However, the postreceptor events that lead to DNA synthesis and cell division have not been well characterized. Previous studies indicate that insulin can regulate the expression of several genes in H4 cells. One of these genes is the proto-oncogene c-fos, a cellular gene whose deregulation has been implicated in the process of cellular differentiation and division. In the present work insulin is shown to regulate cellular c-fos mRNA accumulation and the transcription rate of the c-fos gene. Insulin caused a rapid, dose-dependent increase in the cytoplasmic concentration of c-fos mRNA which was maximal by 30 min. Preceding this, a more rapid 6-8 fold increase in transcription of the c-fos gene was observed. Induction of transcription was apparent following only 5 min of insulin addition. This is the most rapid effect of insulin yet demonstrated on the induction of gene expression. Protein synthesis inhibitors (cycloheximide, anisomycin) also induced the transcription of the c-fos gene. However, they stimulated a much greater increase in transcription than did insulin, and followed a different time course of action...Continue Reading

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.