Intact soil-core microcosms compared with multi-site field releases for pre-release testing of microbes in diverse soils and climates

Canadian Journal of Microbiology
J V GagliardiG Wisse

Abstract

Intact soil-core microcosms were used to compare persistence of Pseudomonas chlororaphis 3732RN-L11 in fallow soil and on wheat roots with field releases at diverse sites. Parallel field and microcosm releases at four sites in 1996 were repeated with addition of one site in 1997. Microcosms were obtained fresh and maintained at 60% soil water holding capacity in a growth chamber at 70% relative humidity, a 12-hour photoperiod, and constant temperature. Persistence of 3732RN-L11 was measured at each site in field plots and microcosms at 7-21 day intervals, and in duplicate microcosms sampled at an independent laboratory. Linear regression slopes of field plot and microcosm persistence were compared for each site, and between identical microcosms sampled at different sites, using log10 transformed plate counts. Microcosm persistence closely matched field plots for wheat roots, but persistence in fallow soil differed significantly in several instances where persistence in field plots was lower than in microcosms. Analysis of weather variations at each site indicated that rainfall events of 30-40 mm caused decreased persistence in fallow soil. Cooler temperatures enhanced persistence in field plots at later time points. Inter-labor...Continue Reading

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Citations

Apr 26, 2002·Environmental Microbiology·Joel V Gagliardi, Jeffrey S Karns
Nov 6, 2004·Applied and Environmental Microbiology·Steven C InghamThomas H Wright
Aug 2, 2007·Canadian Journal of Microbiology·A Mark IbekweChing-Hong Yang
Oct 17, 2006·Journal of Applied Microbiology·C M Ross, A M Donnison
Sep 29, 2019·Plant Disease·Ebrahim OsdaghiCarolee T Bull
Jan 24, 2003·Journal of Food Protection·J V GagliardiD Ingram
Apr 6, 2006·Critical Reviews in Biotechnology·Ajay SinghOwen Ward
Jun 1, 2002·FEMS Microbiology Ecology·James D Nairn, Christopher P Chanway

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