Integral approach to biomacromolecular structure by analytical-ultracentrifugation and small-angle scattering.

Communications Biology
Ken MorishimaMasaaki Sugiyama

Abstract

Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different from the monodispersed one. A concerted method with analytical-ultracentrifugation (AUC) and SAS, named as AUC-SAS, offers the precise scattering intensity of a concerned biomacromolecule in solution even with aggregates as well that of a complex under an association-dissociation equilibrium. AUC-SAS overcomes an aggregation problem which has been an obstacle for SAS analysis and, furthermore, has a potential to lead to a structural analysis for a general multi-component system.

References

Aug 23, 2002·Protein Science : a Publication of the Protein Society·Jacob LebowitzPeter Schuck
Dec 13, 2016·Methods : a Companion to Methods in Enzymology·Trushar R PatelJanusz M Bujnicki
May 5, 2017·Acta Crystallographica. Section D, Structural Biology·Sergei GrudininAndrei Kazennov
Dec 26, 2018·Journal of Biomolecular Structure & Dynamics·Mikhail V KovalchukVladimir I Timofeev

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Methods Mentioned

BETA
X-ray
SANS
size exclusion chromatography

Software Mentioned

SEDNTERP
SEDFIT
AUC
Igor Pro
SAngler
bio
SEDPHAT
SAS
SE

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