May 18, 2018

Integral bHLH factor regulation of cell cycle exit and RGC differentiation

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Kate A MaurerNadean L Brown

Abstract

In the developing mouse embryo, the bHLH transcription factor Neurog2 is transiently expressed by retinal progenitor cells and required for the initial wave of neurogenesis. Remarkably, another bHLH factor, Ascl1, normally not present in the embryonic Neurog2 retinal lineage, can rescue the temporal phenotypes of Neurog2 mutants. Here we show that Neurog2 simultaneously promotes terminal cell cycle exit and retinal ganglion cell differentiation, using mitotic window labeling and integrating these results with retinal marker quantifications. We also analyzed the transcriptomes of E12.5 GFP-expressing cells from Neurog2GFP/+ , Neurog2GFP/GFP , and Neurog2Ascl1KI/GFP eyes, and validated the most significantly affected genes using qPCR assays. Our data support the hypothesis that Neurog2 acts at the top of a retinal bHLH transcription factor hierarchy. The combined expression levels of these downstream factors are sufficiently induced by ectopic Ascl1 to restore RGC genesis, highlighting the robustness of this gene network during retinal ganglion cell neurogenesis. Developmental Dynamics 247:965-975, 2018. © 2018 Wiley Periodicals, Inc.

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Mentioned in this Paper

Biological Markers
Embryo
Real-Time Polymerase Chain Reaction
Ascl1
Genes
Regulation of Biological Process
Entire Embryo
Lactobacillus sp. E12
Ascl1 protein, mouse
ARHGAP4

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