Integral geometry analysis of fluorescence micrographs for quantitative relative comparison of protein adsorption onto polymer surfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Joanna ZemłaJoanna Raczkowska

Abstract

Most methods developed to study protein binding to distinct surfaces can only determine the average amount of adsorbed protein or merely provide (qualitative) information on its spatial distribution. Both these features can be characterized rigorously by integral geometry analysis of fluorescence micrographs. This approach is introduced here to compare the relative protein adsorption onto various polymer surfaces: polystyrene (PS), poly(methyl methacrylate) (PMMA), poly( n-butyl methacrylate) (PnBMA), poly( tert-butyl methacrylate) (PtBMA), and PS(PETA) and cross-linked poly(ethylene oxide) (PEO*(PETA)), admixed with pentaerythritol triacrylate (PETA). The polymeric surfaces were incubated for 15 min in phosphate-buffered saline (pH 7.4) containing 125 mug/mL fluorescently labeled lectins, either lentil lectin (LcH) or concanavalin A (ConA). Fluorescence images were recorded at identical conditions (physiological buffer, same exposure time, magnification, gain). For each image, taken a few times for each polymer, the distribution and average value of the normalized intensity were determined. The results show that the binding of LcH to PS(PETA), PtBMA, PS, PnBMA, PMMA, and PEO*(PETA) can be expressed by the ratio of the followin...Continue Reading

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Citations

Sep 21, 2013·Journal of Colloid and Interface Science·Yurij StetsyshynAndrzej Budkowski
May 7, 2013·ACS Applied Materials & Interfaces·Alicia L GuiJ Justin Gooding
Nov 4, 2011·Colloids and Surfaces. B, Biointerfaces·Joanna ZemłaMałgorzata Lekka
Jul 14, 2010·Colloids and Surfaces. B, Biointerfaces·K AwsiukJ Raczkowska
Sep 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dan LiuJing-Hui Zhang
Aug 28, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Bonnie O LeungAndrew Doran
Mar 16, 2017·ACS Applied Materials & Interfaces·Yurij StetsyshynAndrzej Budkowski

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