Abstract
To evaluate whether tumour cells can be detected in bladder lavage fluid samples by an objective spectrofluorometric method, as 5-aminolaevulinic acid (ALA)-induced fluorescence endoscopy (AFE) and cytology are promising valuable tools for detecting transitional cell carcinoma of the urinary bladder (TCCB). After instilling ALA into the urinary bladder, lavage samples were collected and their sediments analysed spectroscopically under blue excitation at approximately 400 nm wavelength. During AFE, biopsies were taken. From 62 cases, 24 patients had a histologically confirmed TCCB (group A), 28 had a history of TCCB but no evidence of disease (group B) and 10 were negative for TCCB (group C). Lavage sediments of all patients fluoresced in the green spectral range, typical of cellular autofluorescence. Sediments of all patients of group A caused red fluorescence peaking at 635 nm, indicating protoporphyrin IX (PPIX). The PPIX signals derived from bleaching spectra were significantly different between benign and malignant findings (P = 0.001). There was another red fluorescence peak at approximately 620 nm; in 19 cases its intensity exceeded the intensity of the PPIX signal. Spectrofluorometric analysis of lavage sample sediments ...Continue Reading
References
Jun 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·J C KennedyD C Pross
Nov 1, 1994·Urology·P SteinbachR Knüchel
Dec 1, 1994·Urology·M KriegmairA Hofstetter
Nov 1, 1995·Photochemistry and Photobiology·P SteinbachR Knüchel
Oct 29, 1998·British Journal of Cancer·S N DattaP N Matthews
Apr 23, 1999·Journal of Endourology·T FilbeckW F Wieland
Aug 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·K SzocsJ Fidy
Aug 18, 2000·Photochemistry and Photobiology·R C KriegR Knuechel
Apr 3, 2001·Photochemistry and Photobiology·S L GibsonR Hilf
Feb 9, 2002·Urology·Akos Pytel, Nikolaus Schmeller
Apr 16, 2002·Current Opinion in Urology·Rabi Tiguert, Yves Fradet
Sep 7, 2002·Cancer·Dirk ZaakRuth Knöchel
Nov 14, 2002·European Urology·Marie-Ange D'HallewinFrançois Guillemin
May 9, 2003·Urology·Stephan TauberAlfons Hofstetter
Aug 15, 2003·Oncogene·Lior GreenbaumZvi Malik
Jun 22, 2005·European Urology·Shahrokh F ShariatSeth P Lerner
Citations
Jul 20, 2007·Der Urologe. Ausg. A·S TritschlerC G Stief
Jul 21, 2007·Der Urologe. Ausg. A·S Tauber
Aug 19, 2011·Der Urologe. Ausg. A·S TauberM Vierbuchen
Aug 5, 2014·BMC Urology·Yasushi NakaiKiyohide Fujimoto
Dec 22, 2007·European Urology·Dieter JochamRaphaela Waidelich
Mar 8, 2014·Cancer Science·Makito MiyakeKiyohide Fujimoto
Jan 18, 2015·Photodiagnosis and Photodynamic Therapy·Yasushi NakaiKiyohide Fujimoto
Jun 10, 2016·Medical Physics·Brian W PogueJoachim Feldwisch
Jun 12, 2020·Diagnostics·Kit Man ChanMelanie MacGregor
Jul 24, 2021·Indian Journal of Surgical Oncology·Rangrez ShadabChandra Shreya