Integral spectrophotometric analysis of 5-aminolaevulinic acid-induced fluorescence cytology of the urinary bladder

BJU International
Stephan TauberChristian Stief

Abstract

To evaluate whether tumour cells can be detected in bladder lavage fluid samples by an objective spectrofluorometric method, as 5-aminolaevulinic acid (ALA)-induced fluorescence endoscopy (AFE) and cytology are promising valuable tools for detecting transitional cell carcinoma of the urinary bladder (TCCB). After instilling ALA into the urinary bladder, lavage samples were collected and their sediments analysed spectroscopically under blue excitation at approximately 400 nm wavelength. During AFE, biopsies were taken. From 62 cases, 24 patients had a histologically confirmed TCCB (group A), 28 had a history of TCCB but no evidence of disease (group B) and 10 were negative for TCCB (group C). Lavage sediments of all patients fluoresced in the green spectral range, typical of cellular autofluorescence. Sediments of all patients of group A caused red fluorescence peaking at 635 nm, indicating protoporphyrin IX (PPIX). The PPIX signals derived from bleaching spectra were significantly different between benign and malignant findings (P = 0.001). There was another red fluorescence peak at approximately 620 nm; in 19 cases its intensity exceeded the intensity of the PPIX signal. Spectrofluorometric analysis of lavage sample sediments ...Continue Reading

References

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May 9, 2003·Urology·Stephan TauberAlfons Hofstetter

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Citations

Jul 20, 2007·Der Urologe. Ausg. A·S TritschlerC G Stief
Dec 22, 2007·European Urology·Dieter JochamRaphaela Waidelich
Jun 12, 2020·Diagnostics·Kit Man ChanMelanie MacGregor
Jul 24, 2021·Indian Journal of Surgical Oncology·Rangrez ShadabChandra Shreya

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