Integrase mediates nuclear localization of Ty3

Molecular and Cellular Biology
S S LinSuzanne B Sandmeyer

Abstract

Retroviruses in nondividing cells and yeast retrotransposons must transit the nuclear membrane in order for integration to occur. Mutations in a bipartite basic motif in the carboxyl-terminal domain of the Ty3 integrase (IN) protein were previously shown to block transposition at a step subsequent to 3'-end processing of Ty3 extrachromosomal DNA. In this work, the Ty3 IN was shown to be sufficient to target green fluorescent protein to the nucleolus. Mutations in the bipartite basic motif abrogated this localization. The region containing the motif was shown to be sufficient for nuclear but not subnuclear localization of a heterologous protein. Viruslike particles (VLPs) from cells expressing a Ty3 element defective for nuclear localization were inactive in an in vitro integration assay, suggesting that nuclear entry is required to form active VLPs or that this motif is required for post-nuclear entry steps. Ty3 inserts at transcription initiation sites of genomic tRNA genes and plasmid-borne 5S and U6 RNA genes transcribed by RNA polymerase III. In situ hybridization with Ty3- and Ty3 long terminal repeat-specific probes showed that these elements which are associated with tRNA genes do not colocalize with the ribosomal DNA (r...Continue Reading

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Citations

Apr 20, 2005·Genome Research·Becky IrwinSuzanne Sandmeyer
Sep 18, 2009·Journal of Virology·Nadejda Beliakova-BethellSuzanne Sandmeyer
Feb 28, 2002·Journal of Virology·M Henrietta Nymark-McMahonSuzanne B Sandmeyer
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Evan A Farkash, Eline T Luning Prak
Dec 8, 2004·Genetics·Michael AyeSuzanne Sandmeyer
Mar 27, 2003·Traffic·Urs F Greber, Ariberto Fassati
Oct 1, 2015·PLoS Genetics·Virginia BilanchoneSuzanne Sandmeyer
Mar 14, 2017·Nature Reviews. Genetics·Tania SultanaPascale Lesage
Jun 30, 2019·Genome Research·Kurt PattersonSuzanne B Sandmeyer
Jun 6, 2017·Mobile Genetic Elements·Tabitha J McCullers, Mindy Steiniger
Apr 26, 2018·PLoS Genetics·Paul A RowleySara L Sawyer
Jun 25, 2015·Microbiology Spectrum·Suzanne SandmeyerVirginia Bilanchone
Oct 9, 2002·Genome Research·Prescott L Deininger, Mark A Batzer

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