Abstract
Miniature pigs, a valuable alternative model for understanding human tooth development, have deciduous teeth from all four tooth families that are replaced once by permanent molars. The extracellular matrix (ECM) supports cells and maintains the integrity of tooth germs during tooth development. However, details on the role of the ECM in tooth development are poorly understood. Here, we performed long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the ECM components of deciduous tooth germs by RNA sequencing in miniature pigs. From the early cap to the late bell stages, we identified 4,562 and 3,238 differentially expressed genes (DEGs) from E40 to E50 and E50 to E60, respectively. In addition, a total of 1,464 differentially expressed lncRNAs from E40 to E50 and 969 differentially expressed lncRNAs from E50 to E60 were obtained. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs were enriched significantly for multiple signaling pathways, especially for the ECM pathway. We then outlined the detailed dynamic gene expression profiling of ECM components during deciduous molar development. Comparison of the cap and bell stages revealed that the structure and functions of the EC...Continue Reading
References
Mar 8, 2000·Mechanisms of Development·J Jernvall, I Thesleff
Oct 20, 2007·Oral Diseases·S WangS Shi
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Mar 3, 2012·Nature Protocols·Cole TrapnellLior Pachter
Mar 30, 2013·Orthodontic Waves : Official Journal of the Japanese Orthodontic Society·Keigo Yoshizaki, Yoshihiko Yamada
May 18, 2013·Oral Diseases·F WangS Wang
Nov 19, 2013·Australian Dental Journal·I Thesleff
Dec 4, 2013·Nature Reviews. Genetics·Alessandro Fatica, Irene Bozzoni
Apr 23, 2014·BMC Developmental Biology·Tieli SongSonglin Wang
May 3, 2014·BioMed Research International·Rui DongZhipeng Fan
Aug 26, 2014·Nature Biotechnology·Charles WangWeida Tong
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Nov 6, 2014·Nature Reviews. Molecular Cell Biology·Janna K MouwValerie M Weaver
Jan 20, 2015·Nature Genetics·Matthew K IyerArul M Chinnaiyan
Mar 6, 2015·Current Stem Cell Research & Therapy·Ruoshi XuXuedong Zhou
Nov 1, 2015·Methods in Molecular Biology·Sreya Ghosh, Chon-Kit Kenneth Chan
Nov 22, 2015·Current Topics in Developmental Biology·Anamaria Balic, Irma Thesleff
Jan 28, 2016·Genome Biology·Ana ConesaAli Mortazavi
Mar 18, 2016·Archives of Oral Biology·Yunfei Zheng, Lingfei Jia
Sep 15, 2016·Cells, Tissues, Organs·Qiao YiZhipeng Fan
Nov 4, 2016·Nature·Jesse M EngreitzEric S Lander
Jan 4, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Ambra PozziRenato V Iozzo
Feb 12, 2017·BMC Genomics·Fu WangSonglin Wang
Nov 14, 2017·Journal of Proteomics·Yang LiSonglin Wang
Jun 2, 2018·Current Topics in Developmental Biology·Jonathon M Muncie, Valerie M Weaver
Aug 4, 2018·Frontiers in Physiology·Yuanyuan WangPetros Papagerakis