Integrated analysis of microRNA regulatory network in nasopharyngeal carcinoma with deep sequencing

Journal of Experimental & Clinical Cancer Research : CR
Fan WangBao Zhang

Abstract

MicroRNAs (miRNAs) have been shown to play a critical role in the development and progression of nasopharyngeal carcinoma (NPC). Although accumulating studies have been performed on the molecular mechanisms of NPC, the miRNA regulatory networks in cancer progression remain largely unknown. Laser capture microdissection (LCM) and deep sequencing are powerful tools that can help us to detect the integrated view of miRNA-target network. Illumina Hiseq2000 deep sequencing was used to screen differentially expressed miRNAs in laser-microdessected biopsies between 12 NPC and 8 chronic nasopharyngitis patients. The result was validated by real-time PCR on 201 NPC and 25 chronic nasopharyngitis patients. The potential candidate target genes of the miRNAs were predicted using published target prediction softwares (RNAhybrid, TargetScan, Miranda, PITA), and the overlay part was analyzed in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological process. The miRNA regulatory network analysis was performed using the Ingenuity Pathway Analysis (IPA) software. Eight differentially expressed miRNAs were identified between NPC and chronic nasopharyngitis patients by deep sequencing. Further qRT-PCR assays confirmed 3 d...Continue Reading

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Citations

Aug 26, 2016·Journal of Human Genetics·Keiichi KoshizukaNaohiko Seki
Dec 7, 2016·Journal of Experimental & Clinical Cancer Research : CR·Teng HuangXia He
May 9, 2018·Angiogenesis·Anna Janaszak-JasieckaRafal Bartoszewski
Aug 9, 2016·Journal of Biochemical and Molecular Toxicology·Hong-Ling YanWei Xu
Sep 27, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying-Juan ZhengZhang-Suo Liu
May 7, 2020·Histochemistry and Cell Biology·Alexandra PoteAna Marques-Ramos
Oct 7, 2017·Journal of Experimental & Clinical Cancer Research : CR·Ning JiangXia He
Aug 29, 2019·Frontiers in Oncology·Sumei WangWanyin Wu
May 28, 2019·The Journal of International Medical Research·Yu-Zhong XuChen He
Apr 12, 2020·International Journal of Molecular Sciences·Omid KooshkakiBehzad Baradaran

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Datasets Mentioned

BETA
289899

Methods Mentioned

BETA
RNA-Seq
laser capture microdissection
biopsy
reverse
reverse transcription-PCR
PCR
transfection

Clinical Trials Mentioned

NCT01171235

Software Mentioned

IPA
Miranda
RNAhybrid
PITA
TargetScan

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