Integrated analysis of sialotranscriptome and sialoproteome of the brown dog tick Rhipicephalus sanguineus (s.l.): Insights into gene expression during blood feeding.

Journal of Proteomics
Lucas TirloniJ M C Ribeiro

Abstract

Tick salivary glands secrete a complex saliva into their hosts which modulates vertebrate hemostasis, immunity and tissue repair mechanisms. Transcriptomic studies revealed a large number of transcripts coding for structural and secreted protein products in a single tick species. These transcripts are organized in several large families according to their products. Not all transcripts are expressed at the same time, transcription profile switches at intervals, characterizing the phenomenon of "sialome switching". In this work, using transcriptomic and proteomic analysis we explored the sialome of Rhipicephalus sanguineus (s.l.) adult female ticks feeding on a rabbit. The correlations between transcriptional and translational results in the different groups were evaluated, confirming the "sialome switching" and validating the idea that the expression switch may serve as a mechanism of escape from the host immunity. Recombination breakpoints were identified in lipocalin and metalloprotease families, indicating this mechanism could be a possible source of diversity in the tick sialome. Another remarkable observation was the identification of host-derived proteins as a component of tick salivary gland content. These results and dis...Continue Reading

Citations

Nov 3, 2020·Parasite Immunology·Larissa Almeida MartinsMichail Kotsyfakis
Jan 12, 2021·Parasite Immunology·Yu Quan NgPeter J Krause
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Lucas TirloniItabajara da Silva Vaz
Jun 13, 2021·The Journal of Biological Chemistry·Stepan S DenisovIngrid Dijkgraaf

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