Integrated Chemical and Transcriptomic Analysis Reveals the Distribution of Protopanaxadiol- and Protopanaxatriol-Type Saponins in Panax notoginseng

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Guangfei WeiShilin Chen

Abstract

Panax notoginseng is famous for its important therapeutic effects and commonly used worldwide. The active ingredients saponins have distinct contents in different tissues of P. notoginseng, and they may be related to the expression of key genes in the synthesis pathway. In our study, high-performance liquid chromatography results indicated that the contents of protopanaxadiol-(Rb1, Rc, Rb2, and Rd) and protopanaxatriol-type (R1, Rg1, and Re) saponins in below ground tissues were higher than those in above ground tissues. Clustering dendrogram and PCA analysis suggested that the below and above ground tissues were clustered into two separate groups. A total of 482 and 882 unigenes were shared in the below and above ground tissues, respectively. A total of 75 distinct expressions of CYPs transcripts (RPKM ≥ 10) were detected. Of these transcripts, 38 and 37 were highly expressed in the below ground and above ground tissues, respectively. RT-qPCR analysis showed that CYP716A47 gene was abundantly expressed in the above ground tissues, especially in the flower, whose expression was 31.5-fold higher than that in the root. CYP716A53v2 gene was predominantly expressed in the below ground tissues, especially in the rhizome, whose expre...Continue Reading

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Citations

Jan 29, 2021·Toxicon : Official Journal of the International Society on Toxinology·Madiha ZaynabShuangfei Li
Jun 4, 2021·Microbial Biotechnology·Guangfei WeiShilin Chen

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Datasets Mentioned

BETA
SRR7427745-SRR7427748

Methods Mentioned

BETA
PCA
transgenic
electrophoresis
PCR

Software Mentioned

BLASTX
SPSS
R
Trinity
BLAST

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