Integrated effects of multiple modulators on human liver glycogen phosphorylase a

American Journal of Physiology. Endocrinology and Metabolism
N Ercan-FangF Q Nuttall

Abstract

Hepatic glucose production is increased in people with type 2 diabetes. Glucose released from storage in liver glycogen by phosphorylase accounts for approximately 50% of the glucose produced after an overnight fast. Therefore, understanding how glycogenolysis in the liver is regulated is of great importance. Toward this goal, we have determined the kinetic characteristics of recombinant human liver glycogen phosphorylase a (HLGPa) (active form) and compared them with those of the purified rat enzyme (RLGPa). The Michaelis-Menten constant (K(m)) of HLGPa for P(i), 5 mM, was about fivefold greater than the K(m) of RLGPa. Two P(i) (substrate) concentrations were used (1 and 5 mM) to cover the physiological range for P(i). Other effectors were added at estimated intracellular concentrations. When added individually, AMP stimulated, whereas ADP, ATP and glucose inhibited, activity. These results were similar to those of the RLGPa. However, glucose inhibition was about twofold more potent with the human enzyme. UDP-glucose, glucose 6-phosphate, and fructose 1-phosphate were only minor inhibitors of both enzymes. We reported previously that when all known effectors were present in combination at physiological concentrations, the net ...Continue Reading

References

Jan 1, 1978·Current Topics in Cellular Regulation·G Van den Berghe
Dec 1, 1976·The Biochemical Journal·B Lederer, W Stalmans
Apr 6, 1992·Biochimica Et Biophysica Acta·K SchiebelD Mayer
Mar 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L N Johnson
Feb 1, 1992·Archives of Biochemistry and Biophysics·S A Westphal, F Q Nuttall
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A P KoretskyT Van Dyke
Feb 9, 1990·Molecular and Cellular Biochemistry·W J RoeslerR L Khandelwal
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·C B NewgardR J Fletterick
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C B NewgardR J Fletterick
Jan 1, 1986·Advances in Experimental Medicine and Biology·G Van den Berghe, J Jaeken
Jan 1, 1987·Diabetes/metabolism Reviews·G van de Werve, B Jeanrenaud
Apr 1, 1987·Metabolism: Clinical and Experimental·C B NiewoehnerF Q Nuttall
Nov 28, 1988·The American Journal of Medicine·F Q NuttallA W Tan
Jul 1, 1971·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·C BodeH Pelzel
May 22, 1970·Biochemical and Biophysical Research Communications·W GlinsmannE Hern
Jan 1, 1980·Physiological Reviews·D A Hems, P D Whitton
Jan 1, 1984·The American Journal of Physiology·C B NiewoehnerF Q Nuttall
Oct 1, 1984·The American Journal of Physiology·C B NiewoehnerF Q Nuttall
Jan 1, 1980·Annual Review of Biochemistry·R J Fletterick, N B Madsen
Mar 1, 1981·The Biochemical Journal·G Gevers, W Stalmans
Oct 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R BuchliP Boesiger
Dec 5, 1993·Journal of Molecular Biology·J W HudsonM M Crerar
Apr 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KeppensH De Wulf
Apr 15, 1996·Archives of Biochemistry and Biophysics·N ErcanF Q Nuttall
Jan 1, 1996·The American Journal of Physiology·K F PetersenG I Shulman
Sep 1, 1997·The Journal of Clinical Investigation·M K HellersteinA Letscher
Nov 10, 1998·The Biochemical Journal·M BollenW Stalmans
Sep 14, 1999·The American Journal of Physiology·R M O'DohertyC B Newgard
Sep 12, 2000·Chemistry & Biology·V L RathD J Hoover
Dec 20, 2000·American Journal of Physiology. Endocrinology and Metabolism·G BodenT P Stein
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·N Ercan-FangMary C Gannon

Citations

Aug 8, 2009·The Journal of Clinical Investigation·Eric D BerglundDavid H Wasserman
Nov 18, 2006·Annals of Biomedical Engineering·J KimMarco E Cabrera
Jan 7, 2010·Expert Opinion on Investigational Drugs·Ikna Espinosa, Leon Fogelfeld
Jul 4, 2012·Molecular and Cellular Endocrinology·Jeevan NagendranJason R B Dyck
Apr 7, 2016·BBA Clinical·María M Adeva-AndanyEva Ameneiros-Rodríguez
Oct 11, 2014·PloS One·Ildefonso M De la FuenteLuis Martínez
Oct 19, 2007·Diabetes, Obesity & Metabolism·Y LuT W Donner
Oct 6, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gülin OzElizabeth R Seaquist
Mar 1, 2018·The Journal of Biological Chemistry·J Scott LeeSean P Colgan
Oct 19, 2018·Journal of Food Science·Sohini RoyAshis Kumar Samanta
Mar 31, 2005·American Journal of Physiology. Endocrinology and Metabolism·N Ercan-FangF Q Nuttall
Oct 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Raul C CamachoDavid H Wasserman
Jun 15, 2006·American Journal of Physiology. Endocrinology and Metabolism·Michael ChristopherFrank P Alford
Nov 30, 2006·American Journal of Physiology. Endocrinology and Metabolism·Gülin OzRolf Gruetter
Jan 27, 2005·American Journal of Physiology. Endocrinology and Metabolism·Masakazu ShiotaAlan D Cherrington
Mar 28, 2006·The Journal of Biological Chemistry·Olivera GrubishaJohn M Denu
Apr 5, 2003·American Journal of Physiology. Endocrinology and Metabolism·Tony K T LamAdria Giacca
Jul 28, 2005·American Journal of Physiology. Endocrinology and Metabolism·Raul C CamachoDavid H Wasserman

Related Concepts

fructose-1-phosphate, monosodium salt, (D)-isomer
Magnesium ADP
Phosphaden
Adenosine Triphosphate, Chromium Ammonium Salt
Enzyme Activation
Enzyme Inhibitors
Fructosephosphates
Glucose, (beta-D)-Isomer
Liver
Orthophosphate

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.