PMID: 11320511Apr 26, 2001Paper

Integrated flow-injection processing for on-line quantification of plasmid DNA during cultivation of E. coli

Biotechnology and Bioengineering
M P NandakumarB Mattiasson

Abstract

An integrated flow-injection processing (FIP) system for the quantification of plasmids during cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an integrated manner during cultivation of E. coli. The system was operated by using a miniaturized expanded-bed column which can be used for handling samples containing cells and cell debris without interfering with the binding analysis. Two types of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed using standard solutions and it was successfully applied in monitoring plasmid contents during a cultivation of E. coli.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jun 5, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J ZhangF Regnier
Nov 26, 1998·Journal of Biotechnology·A GillM Hoare
Jun 7, 2000·Bioseparation·G N FerreiraD M Prazeres
Jan 20, 1995·Biotechnology and Bioengineering·M KyröläinenB Mattiasson

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Citations

Jun 22, 2005·Biosensors & Bioelectronics·Martin HedströmBo Mattiasson

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