Integrated genome-based probiotic relevance and safety evaluation of Lactobacillus reuteri PNW1

PloS One
Kazeem Adekunle AlayandeCollins Njie Ateba

Abstract

This study evaluates whole-genome sequence of Lactobacillus reuteri PNW1 and identifies its safety genes that may qualify it as a putative probiotic. It further extracted the bacteriocin produced by the strain and tested its effectiveness against pathogenic STEC E. coli O177. The genomic DNA was sequenced on illuminal Miseq instrument and the sequenced data was assessed for quality reads before assembled with SPAdes. The draft assembly was annotated with Prokaryotic Genome Annotation Pipeline (PGAP) and Rapid Annotations using Subsystems Technology (RAST). Further downstream analyses were carried out using appropriate bioinformatic tools. Production of biogenic amines was biochemically confirmed through HPLC analysis. The assembled genome was 2,430,215 bp long in 420 contigs with 39% G+C content. Among all known genes, putatively responsible for the production of toxic biochemicals, only arginine deiminase (EC3.5.3.6) was spotted. Coding sequences (CDS) putative for D-lactate dehydrogenase (EC1.1.1.28), L-lactate dehydrogenase (EC1.1.1.27) and bacteriocin helveticin J were found within the genome together with plethora of other probiotic important genes. The strain harbours only resistant genes putative for Lincosamide (lnuC) a...Continue Reading

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Citations

Oct 3, 2020·Microbiology Resource Announcements·Mpinda Edoaurd TshipambaMulunda Mwanza
Oct 3, 2020·Microbiology Resource Announcements·Mpinda Edoaurd TshipambaMulunda Mwanza
Aug 18, 2021·Probiotics and Antimicrobial Proteins·Luís Cláudio Lima de JesusVasco Azevedo
Aug 25, 2021·Journal of Industrial Microbiology & Biotechnology·Alejandra Mejía CaballeroAmelia Farrés
Oct 6, 2021·Pharmacology Research & Perspectives·María Elena Fátima Nader-MacíasJessica Alejandra Silva

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Datasets Mentioned

BETA
RJWE00000000
PRJNA504734

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
ISfinder
Optimised Annotation System for Insertion Sequences ( OASIS )
blastx
SEED viewer
ResFinder
PathogenFinder
CRISPRCasFinder
Phage Search Tool Enhanced Release ( PHASTER )
ISsaga

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