Integrated Graphene Oxide Purification-Lateral Flow Test Strips (iGOP-LFTS) for Direct Detection of PCR Products with Enhanced Sensitivity and Specificity

Analytical Chemistry
Shanglin LiPeng Liu

Abstract

An integrated graphene oxide purification-lateral flow test strip (iGOP-LFTS) was developed for on-strip purifying and visually detecting polymerase chain reaction (PCR) products with an improved sensitivity as well as a more stringent specificity. PCR products amplified with a pair of biotin- and digoxin-labeled primers were directly pipetted onto GO pads, on which graphene oxide selectively adsorbed residual primers and primer-dimers with the aid of a running buffer containing MgCl2 and Tween 20. By stacking up three GO pads to increase the surface area for adsorption, 83.4% of double-stranded DNA with a length of 30 bp and 98.6% of 20-nt primers could be removed from a 10-μL DNA mixture. Since no primers interfered with detection, the increase of the sample loading volume from 5 to 20 μL could improve the signal-to-noise ratio of the test line 1.6 fold using the iGOP-LFTS while no changes were observed using the conventional LFTS. The limit of detection of the iGOP-LFTS was determined to be 30 copies of bacteriophage λ-DNA with naked eyes and this limit could be further decreased to 3 copies by loading 20 μL of the sample, which corresponded to a 1000-fold improvement compared to that of the LFTS detected by naked eyes. When...Continue Reading

References

Dec 16, 1998·Nature Biotechnology·P RossL Haff
Jul 6, 2000·Applied and Environmental Microbiology·D KozwichP A Roehl
Jan 26, 2008·Chemical Communications : Chem Comm·Jenny AveyardRobert Wilson
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
May 29, 2009·Angewandte Chemie·Chun-Hua LuGuo-Nan Chen
Mar 17, 2010·The Journal of Infectious Diseases·Christopher D PilcherMatthew Golden
Jun 27, 2012·Lab on a Chip·Brittany A Rohrman, Rebecca R Richards-Kortum
Jun 30, 2012·Chemical Communications : Chem Comm·Shiva K RastogiPatrick J Hrdlicka
Apr 29, 2014·Biosensors & Bioelectronics·Li GaoKeping Chen
Jun 21, 2014·Biosensors & Bioelectronics·Yong Tae KimTae Seok Seo
Sep 15, 2014·Biosensors & Bioelectronics·Jia Li, Joanne Macdonald
Oct 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joon Soo ParkDong-Eun Kim
Dec 4, 2014·Analytica Chimica Acta·Huimin Deng, Zhiqiang Gao
Jun 27, 2015·Chemical Reviews·Wen ZhouXiaoyuan Chen
Dec 15, 2015·Biosensors & Bioelectronics·Xiao-Jing XingHong-Wu Tang
Jan 5, 2016·Journal of Nanoscience and Nanotechnology·Seyeon KimJongback Gang
May 18, 2016·Nature Reviews. Genetics·Sara GoodwinW Richard McCombie
Nov 24, 2016·Diagnostics·Mílton CordeiroPedro Viana Baptista

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Citations

Oct 17, 2018·Sensors·Shrikant Dashrath WarkadTaisun Kim
Aug 8, 2020·Biosensors & Bioelectronics·Jeanne E van DongenLoes I Segerink
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yogesh Kumar, Kairam Narsaiah
May 20, 2021·Journal of Agricultural and Food Chemistry·Panting SangHe Qian
Nov 24, 2020·ACS Sensors·Cécilia Ménard-MoyonKourosh Kalantar-Zadeh

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