Integrated HPV genomes tend to integrate in gene desert areas in the CaSki, HeLa, and SiHa cervical cancer cell lines

Life Sciences
Ming-Kun DiaoYi Zeng

Abstract

The integration preferences of human papillomavirus (HPV) have been intensively studied and contested over recent years. To disclose the integration preferences of high-risk HPV in cervical cancer, HPV transcriptional sites and features in different cervical cancer cell lines were identified. In this study, three cervical cancer cell lines (CaSki, HeLa, and SiHa) were subjected for HPV genome status determination by amplification of papillomavirus oncogene transcripts (APOT) assay. The numbers of viral copies in human genomes and numbers of viral-human fusion mRNAs in three HPV-integrated cervical cancer cell lines were measured and analysed. The results revealed that the gene desert region 8q24 of the HPV type 18 integrated HeLa cell line and the 13q21-22 region of the HPV type 16 integrated CaSki and SiHa cell lines were hotspots for HPV integration, and the numbers of viral copies in the human genomes of the three cell lines that we detected were not in accordance with those reported in previous studies. Integration of the HPV genome into the host cell chromosome suggests that persistent HPV infection is vital for malignant cell transformation and carcinogenesis. This study provides information to benefit health care profess...Continue Reading

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Citations

Sep 4, 2015·Scientific Reports·Sen DingPingping Shen
Aug 1, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Yuniardini Septorini WimardhaniAnak Iamaroon
Apr 23, 2020·International Journal of Oncology·Pedro Rosendo-ChalmaAlejandro García-Carrancá
Apr 22, 2021·Annual Review of Virology·Christian K PfallerCharles E Samuel
Dec 25, 2021·Frontiers in Cellular and Infection Microbiology·Xinxin XuFang Li

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