Integrated kinetic and probabilistic modeling of the growth potential of bacterial populations

Applied and Environmental Microbiology
Susan M GeorgeJ Baranyi

Abstract

When bacteria are exposed to osmotic stress, some cells recover and grow, while others die or are unculturable. This leads to a viable count growth curve where the cell number decreases before the onset of the exponential growth phase. From such curves, it is impossible to estimate what proportion of the initial cells generates the growth because it leads to an ill-conditioned numerical problem. Here, we applied a combination of experimental and statistical methods, based on optical density measurements, to infer both the probability of growth and the maximum specific growth rate of the culture. We quantified the growth potential of a bacterial population as a quantity composed from the probability of growth and the "suitability" of the growing subpopulation to the new environment. We found that, for all three laboratory media studied, the probability of growth decreased while the "work to be done" by the growing subpopulation (defined as the negative logarithm of their suitability parameter) increased with NaCl concentration. The results suggest that the effect of medium on the probability of growth could be described by a simple shift parameter, a differential NaCl concentration that can be accounted for by the change in the ...Continue Reading

References

Nov 1, 1994·International Journal of Food Microbiology·J Baranyi, T A Roberts
Jan 30, 1999·Applied and Environmental Microbiology·J Baranyi, C Pin
Aug 12, 1999·International Journal of Food Microbiology·J BaranyiT Ross
Apr 6, 2002·International Journal of Food Microbiology·Y Le MarcD Thuault
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May 15, 2003·International Journal of Food Microbiology·L A MellefontT Ross
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Jan 14, 2004·Journal of Food Protection·Konstantinos P KoutsoumanisJohn N Sofos
Sep 30, 2004·Journal of Food Protection·József Baranyi, Mark L Tamplin
Mar 24, 2009·Applied and Environmental Microbiology·Claire Dupont, Jean-Christophe Augustin
Jan 5, 2011·Applied and Environmental Microbiology·K ZhouJ Baranyi
Aug 3, 2011·International Journal of Food Microbiology·Aline MetrisJózsef Baranyi
Dec 3, 2013·International Journal of Food Microbiology·József BaranyiJudit Beczner
May 27, 2014·Applied and Environmental Microbiology·A MetrisJ Baranyi

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environmental stress
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