Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana

Chinese Medicine
Jiang HeGuan Li

Abstract

Ziziphora bungeana Juz. is a folk medicine from the Xinjiang Uygur Autonomous Region. The herb or the aerial parts of it have been used to medicinally treat cardiovascular diseases. Flavonoids are the main pharmacologically active ingredients in Z. bungeana. Identification of the tissue-specific distribution of flavonoids in Z. bungeana is crucial for effective and sustainable medicinal use of the plant. Furthermore, understanding of the biosynthesis pathways of these flavonoids in Z. bungeana is of great biological significance. The flavonoids from different tissues of Z. bungeana were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The full-length transcriptome of Z. bungeana was determined using a strategy based on a combination of Illumina and PacBio sequencing techniques. The functions of differentially expressed unigenes were predicted using bioinformatics methods and further investigated by real-time quantitative PCR and phylogenetic relationship analysis. Among the 12 major flavonoid components identified from Z. bungeana extracts, linarin was the most abundant component. Nine flavonoids were identified as characteristic components of specific tissues. Transcriptome profiling and bioinformati...Continue Reading

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Datasets Mentioned

BETA
SRR10352318
SRR10352311
SRR10352310
SRR10352309
SRR10352308
SRR10352307
SRR10352306
SRR10352305
SRR10352304
SRR10352317

Methods Mentioned

BETA
transcriptomic profiling
PCR
Illumina sequencing
glycosylation

Software Mentioned

HIT
tBLASTn
BestKeeper
Evolview
pheatmap
R
GeNorm
Vector NTI
RSEM
CD

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