Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus

Ticks and Tick-borne Diseases
Angélica Hernández-JarguínJosé de la Fuente

Abstract

An innovative metaomics approach integrating metatranscriptomics and metaproteomics was used to characterize bacterial communities in the microbiota of the Lyme borreliosis spirochete vector, Ixodes ricinus (Acari: Ixodidae). Whole internal tissues and salivary glands from unfed larvae and female ticks, respectively were used. Reused I. ricinus RNA-sequencing data for metranscriptomics analysis together with metaproteomics provided a better characterization of tick bacterial microbiota by increasing bacteria identification and support for identified bacteria with putative functional implications. The results showed the presence of symbiotic, commensal, soil, environmental, and pathogenic bacteria in the I. ricinus microbiota, including previously unrecognized commensal and soil microorganisms. The results of the metaomics approach may have implications in the characterization of putative mechanisms by which pathogen infection manipulates tick microbiota to facilitate infection. Metaomics approaches integrating different omics datasets would provide a better description of tick microbiota compositions, and insights into tick interactions with microbiota, pathogens and hosts.

Citations

Mar 10, 2020·Bioengineered·Mukesh Kumar AwasthiZengqiang Zhang
Mar 19, 2020·Cellular Microbiology·Fabrizia StavruDavide Sassera
Jan 16, 2021·Parasites & Vectors·L Paulina Maldonado-RuizLudek Zurek
May 9, 2021·Trends in Parasitology·Sukanya NarasimhanErol Fikrig

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