Integrated microfluidic biochips for DNA microarray analysis

Expert Review of Molecular Diagnostics
Robin Hui LiuAndy McShea

Abstract

A fully integrated and self-contained microfluidic biochip device has been developed to automate the fluidic handling steps required to perform a gene expression study of the human leukemia cell line (K-562). The device consists of a DNA microarray semiconductor chip with 12,000 features and a microfluidic cartridge that consists of microfluidic pumps, mixers, valves, fluid channels and reagent storage chambers. Microarray hybridization and subsequent fluidic handling and reactions (including a number of washing and labeling steps) were performed in this fully automated and miniature device before fluorescent image scanning of the microarray chip. Electrochemical micropumps were integrated in the cartridge to provide pumping of liquid solutions. A micromixing technique based on gas bubbling generated by electrochemical micropumps was developed. Low-cost check valves were implemented in the cartridge to prevent cross-talk of the stored reagents. A single-color transcriptional analysis of K-562 cells with a series of calibration controls (spiked-in controls) was performed to characterize this new platform with regard to sensitivity, specificity and dynamic range. The device detected sample RNAs with a concentration as low as 0.37...Continue Reading

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Citations

Feb 23, 2007·American Journal of Physical Medicine & Rehabilitation·Assaf T GordonRory A Cooper
Nov 20, 2012·Applied and Environmental Microbiology·Darrell P ChandlerPhilip E Long
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Nov 23, 2006·Electrophoresis·Eszter Szántai, András Guttman
Sep 9, 2019··Soumya RadhakrishnanDag Hanstorp

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