Integrated Microfluidic Sample-to-Answer System for Direct Nucleic Acid-Based Detection of Group B Streptococci in Clinical Vaginal/Anal Swab Samples

ACS Sensors
Xin YeJilie Kong

Abstract

The group B Streptococcus (GBS) is a type of pathogen that seriously threatens the health of mothers and infants. Prompt and timely diagnosis is crucial for good patient outcomes. However, the traditional bacterial culture and polymerase chain reaction methods are limited by their speed and involve complex operating procedures. Herein, we successfully established an integrated microfluidic sample-to-answer system for nucleic acid-based detection of GBS directly in vaginal/anal swab samples. Meanwhile, we demonstrated a dynamical reaction mechanism of Bst/FEN1-based nucleic acid amplification, which differs from traditional Bst-based isothermal amplification strategies. The system integrates cell lysis and nucleic acid purification, separation, amplification, and detection, enabling rapid (about 45 min to the entire analysis) and highly accurate (98% accuracy) analysis in a clinical setting. Experimental results show that the system offers a good detection limit (500 CFU/mL), perfect specificity (no cross-reactivity with 25 other common pathogens), excellent stability (coefficient of variation less than 3%), and good anti-interference performance. This novel system holds great potential as a nucleic acid-based diagnostic tool in...Continue Reading

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
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Mar 5, 2013·Annual Review of Biochemistry·Lata Balakrishnan, Robert A Bambara
Nov 26, 2013·Japanese Journal of Infectious Diseases·Kouji KimuraYoshichika Arakawa
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Nov 27, 2016·Biosensors & Bioelectronics·Maria Chiara Giuffrida, Giuseppe Spoto
Apr 25, 2017·Lancet·Andi L ShaneBarbara J Stoll
Nov 17, 2017·The Journal of Pediatrics·Meiwa ToyofukuSatoshi Iwata
Mar 30, 2018·Frontiers in Microbiology·Sarah Shabayek, Barbara Spellerberg
Jan 12, 2019·Obstetrics and Gynecology·Najoua El HelaliAlban Le Monnier

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