Integrated microfluidic systems with sample preparation and nucleic acid amplification

Lab on a Chip
Juxin YinYing Mu

Abstract

Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of diseases and pathogens, yet remains a limiting factor at point of care (POC) treatment. Microfluidic systems are characterized by fast, integrated, miniaturized features which provide an effective platform for qualitative and quantitative detection of nucleic acid molecules. The nucleic acid detection process mainly includes sample preparation and target molecule amplification. Given the advancements in theoretical research and technological innovations to date, nucleic acid extraction and amplification integrated with microfluidic systems has advanced rapidly. The primary goal of this review is to outline current approaches used for nucleic acid detection in the context of microfluidic systems. The secondary goal is to identify new approaches that will help shape future trends at the intersection of nucleic acid detection and microfluidics, particularly with regard to increasing disease and pathogen detection for improved diagnosis and treatment.

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Citations

Feb 14, 2020·Analytical and Bioanalytical Chemistry·Maja SidstedtJohannes Hedman
Dec 22, 2020·Biomicrofluidics·Hsuan-Yu MuJen-Huang Huang
Dec 10, 2020·Science China. Chemistry·Yongxi ZhaoChunhai Fan
May 19, 2021·Lab on a Chip·Francesco Del GiudicePier Luca Maffettone
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Jul 27, 2021·Frontiers in Oncology·Apriliana E R KartikasariMagdalena Plebanski
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia
Jul 27, 2021·Small·Fatemeh NasrollahiAli Khademhosseini

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Methods Mentioned

BETA
nucleic acid amplification
chip
nucleic acid extraction
nucleic
biopsies
PCR
chips
nucleic acids extraction
nucleic acid purification
nucleic acid

Software Mentioned

SIDAO
Atlas
Atlas Genetics io®
dLAMP
GeneXpert
INEAD

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