Integrated morphophysiological assessment of two methods for sperm selection in bovine embryo production in vitro

Theriogenology
A CesariR H Alberio

Abstract

Extensive work was done regarding the ability of Swim up and Percoll gradient to select functional sperm for in vitro embryo production (IVP) systems. The aim of this work was to compare Swim up and Percoll as methods of sperm selection by ultrastructural, biochemical and functional studies. Frozen-thawed semen from two bulls (Experiments 1 and 2, respectively) were treated using Swim up or Percoll discontinuous gradients. Motility, sperm membrane ultrastructure, sperm proteins, in vitro embryo production (insemination doses, cleavage, embryo yield and quality) and embryo sex ratio were scored and compared. Electron transmission microscopy of outer sperm membranes showed higher (P<0.05) percentage of sperm with lost acrosomes in Percoll treated samples compared to Swim up. A differential protein pattern was also detected. When in vitro embryo production was performed, Percoll gradient produced higher (P<0.05) number of fertilizing doses (7.6 versus 5.9, Bull 1; 13.5 versus 7.8, Bull 2) and higher sperm motility (90% versus 76.6%, Bull 1; 81.7% versus 68.3%, Bull 2) than Swim up. The percentage of cleavage (Day 3) was similar in both treatment groups, whereas embryo production rate (Day 7) was higher (39.4% versus 30.2%, Bull 1;...Continue Reading

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Citations

Nov 15, 2006·Reproductive Biology and Endocrinology : RB&E·Marko SamardzijaTomislav Dobranic
Nov 10, 2013·Pakistan Journal of Biological Sciences : PJBS·Nur DuchaMulyoto Pangestu
Jan 19, 2011·Cellular Reprogramming·Joana Claudia MezzaliraMarcelo Bertolini
Aug 24, 2011·Human Fertility : Journal of the British Fertility Society·Xiao-Dong ZhangGuo-Ning Huang
Mar 18, 2010·Animal Reproduction Science·Luiz Sergio Almeida CamargoJoão Henrique Moreira Viana
May 19, 2007·Fertility and Sterility·Andreina CesariMaría G Guevara
Mar 4, 2011·Journal of Veterinary Science·Momena KhatunMohammed Shamsuddin
May 11, 2013·Zygote : the Biology of Gametes and Early Embryos·Michiko NoguchiKazuhiro Kikuchi
Jul 16, 2016·Molecular Human Reproduction·M B Wheeler, M Rubessa
Feb 26, 2019·Biology of Reproduction·Denis A Vaughan, Denny Sakkas
Dec 19, 2019·Frontiers in Cell and Developmental Biology·Iván Oseguera-LópezSerafín Pérez-Cerezales
Mar 6, 2021·Journal of Equine Veterinary Science·Johanna PapinJane M Morrell

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