Integrated Proteome Analysis of the Wheat Embryo and Endosperm Reveals Central Metabolic Changes Involved in the Water Deficit Response during Grain Development

Journal of Agricultural and Food Chemistry
Aiqin GuYueming Yan

Abstract

The embryo and endosperm of wheat have different physiological functions and large differences in protein level. In this study, we performed the first integrated proteome analysis of wheat embryo and endosperm in response to the water deficit during grain development. In total, 155 and 130 differentially expressed protein (DEP) spots in the embryo and endosperm, respectively, were identified by nonlinear two-dimensional electrophoresis and tandem mass spectrometry. These DEPs in the embryo were mainly involved in stress/defense responses such as heat shock-related proteins (HSP) and peroxidase, whereas those in endosperm were mainly related to starch and storage protein synthesis such as α-amylase inhibitor and the globulin-1 S allele. In particular, some storage proteins such as avenin-like proteins and high-molecular weight glutenin subunit Dy12 displayed higher expression levels in the mature endosperm under a water deficit, which might contribute to the improvement in the quality of breadmaking.

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Citations

Mar 14, 2018·Journal of the Science of Food and Agriculture·Jiaxing ZhouYueming Yan
Apr 26, 2018·Frontiers in Plant Science·Xiong DengYueming Yan
Jun 27, 2017·Frontiers in Plant Science·Mingming YangShanshan Wen
Jul 25, 2019·Functional & Integrative Genomics·Mingming YangShanshan Wen

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