Integrated proteomic analysis of tumor necrosis factor α and interleukin 1β-induced endothelial inflammation

Journal of Proteomics
Eelke P BéguinMaartje van den Biggelaar

Abstract

The vascular endothelium provides a unique interaction plane for plasma proteins and leukocytes in inflammation. The pro-inflammatory cytokines Tumor Necrosis Factor α (TNFα) and interleukin 1β (IL-1β) have a profound effect on endothelial cells, which includes increased levels of adhesion molecules and a disrupted barrier function. To assess the endothelial response to these cytokines at the protein level, we evaluated changes in the whole proteome, cell surface proteome and phosphoproteome after 24 h of cytokine treatment. The effects of TNFα and IL-1β on endothelial cells were strikingly similar and included changes in proteins not previously associated with endothelial inflammation. Temporal profiling revealed time-dependent proteomic changes, including a limited number of early responsive proteins such as adhesion receptors ICAM1 and SELE. In addition, this approach uncovered a greater number of late responsive proteins, including proteins related to self-antigen peptide presentation, and a transient increase in ferritin. Peptide-based cell surface proteomics revealed extensive changes at the cell surface, which were in agreement with the whole proteome. In addition, site-specific changes within ITGA5 and ICAM1 were detect...Continue Reading

Citations

Sep 15, 2019·Journal of Separation Science·Yanan LiMingliang Ye
Apr 26, 2020·Molecular & Cellular Proteomics : MCP·Eelke P BéguinMaartje van den Biggelaar
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Eelke P BéguinMaartje van den Biggelaar
Mar 5, 2021·Analytical Chemistry·Yanan LiMingliang Ye
Jun 1, 2021·Oxidative Medicine and Cellular Longevity·Peixi LiuWei Zhu

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