Integrated proteomics, genomics, metabolomics approaches reveal oxalic acid as pathogenicity factor in Tilletia indica inciting Karnal bunt disease of wheat

Scientific Reports
Vishakha PandeyAnil Kumar

Abstract

Tilletia indica incites Karnal bunt (KB) disease in wheat. To date, no KB resistant wheat cultivar could be developed due to non-availability of potential biomarkers related to pathogenicity/virulence for screening of resistant wheat genotypes. The present study was carried out to compare the proteomes of T. indica highly (TiK) and low (TiP) virulent isolates. Twenty one protein spots consistently observed as up-regulated/differential in the TiK proteome were selected for identification by MALDI-TOF/TOF. Identified sequences showed homology with fungal proteins playing essential role in plant infection and pathogen survival, including stress response, adhesion, fungal penetration, invasion, colonization, degradation of host cell wall, signal transduction pathway. These results were integrated with T. indica genome sequence for identification of homologs of candidate pathogenicity/virulence related proteins. Protein identified in TiK isolate as malate dehydrogenase that converts malate to oxaloacetate which is precursor of oxalic acid. Oxalic acid is key pathogenicity factor in phytopathogenic fungi. These results were validated by GC-MS based metabolic profiling of T. indica isolates indicating that oxalic acid was exclusively ...Continue Reading

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Citations

Jun 25, 2019·Briefings in Functional Genomics·Twinkle Chaudhary, Pratyoosh Shukla
Jun 17, 2020·PloS One·Naba Al-SariCristina Legido-Quigley
Aug 25, 2019·Metabolites·Fangfang ChenXiao-Lin Chen
May 15, 2020·Canadian Journal of Microbiology·Daniel F Valenzuela-CotaJosé Á Huerta-Ocampo
Nov 3, 2020·Frontiers in Plant Science·Santosh Kumar BishnoiPawan Kumar Singh

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Methods Mentioned

BETA
electrophoresis
protein folding

Software Mentioned

InterProScan
Flex
Cytoscape
TargetP
MASCOT
ProQ
STRING ( Search Tool for the Retrieval of Interacting Genes )
ScanProsite
ProQ server
SMART

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