Jun 9, 2020Paper

Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2

BioRxiv : the Preprint Server for Biology
Jon Arizti-SanzCameron Myhrvold

Abstract

The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (SHERLOCK and HUDSON Integration to Navigate Epidemics), a sensitive and specific integrated diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We combine the steps of SHERLOCK into a single-step reaction and optimize HUDSON to accelerate viral inactivation in nasopharyngeal swabs and saliva. SHINE's results can be visualized with an in-tube fluorescent readout - reducing contamination risk as amplification reaction tubes remain sealed - and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-PCR with a sample-to-answer time of 50 minutes. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.

Citations

Aug 28, 2020·Nature Biomedical Engineering·Maturada PatchsungChayasith Uttamapinant
Dec 23, 2020·Critical Reviews in Biochemistry and Molecular Biology·Monica C PillonRobin E Stanley
Jan 27, 2021·Biosensors & Bioelectronics·Reza NouriWeihua Guan
Jan 12, 2021·Clinical Biochemistry·Fahreddin PalazMehmet Ozsoz
Nov 11, 2020·Diagnostics·Ngan N T NguyenGulden Camci-Unal
Apr 9, 2021·Transgenic Research·Abozar GhorbaniG Gregory Neely
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Ronghua DingGuangcai Duan
Jul 14, 2021·Advanced Science·Vanja PerčulijaSongying Ouyang

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