Integrated Serum and Fecal Metabolomics Study of Collagen-Induced Arthritis Rats and the Therapeutic Effects of the Zushima Tablet

Frontiers in Pharmacology
Jinjun ShanLiuqing Di

Abstract

The Zushima tablet (ZT) has been used for decades in the clinical treatment of rheumatoid arthritis (RA) in China. However, its therapeutic mechanism is unclear. In this study, we aimed to explore the distinctive metabolic patterns in collagen-induced arthritis (CIA) rats and evaluate the therapeutic effects of ZT on RA using untargeted serum and fecal metabolomics approaches based on gas chromatography coupled with mass spectrometry. Body weight, hind paw swelling, TNF-α and IL-1β levels, arthritis scores, and histopathological parameters were assessed. In the metabolomics study, 31 altered metabolites in the serum and 30 in the feces were identified by comparing the model with the control group using statistical processing. These altered metabolites revealed that the tricarboxylic acid cycle, glycolysis metabolism, fatty acid metabolism, and purine metabolism were disturbed in CIA rats, and most of these altered metabolites including l-isoleucine, l-aspartic acid, pyruvic acid, cholic acid, and hypoxanthine, were rectified by ZT. Furthermore, short-chain fatty acids in feces were quantitatively determined, and the results showed that ZT could regulate the levels of propionate, butyrate, and valerate in CIA rats. Then, gut mic...Continue Reading

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Citations

Sep 24, 2020·Mediators of Inflammation·Huihui XuQingdong Guan
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Shaobao ZhangZhiyong Xie
Mar 9, 2020·Ecotoxicology and Environmental Safety·Hui YuYing Zhang
May 31, 2020·Joint, Bone, Spine : Revue Du Rhumatisme·Cheng LiZhi-Ling Sun

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Methods Mentioned

BETA
ELISA
PCR
PCA

Software Mentioned

MS
DIAL
UPARSE
MetaboAnalyst

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