Integrating Cellular and Bioprocess Engineering in the Non-Conventional Yeast Yarrowia lipolytica for Biodiesel Production: A Review

Frontiers in Bioengineering and Biotechnology
Dongming Xie

Abstract

As one of the major biofuels to replace fossil fuel, biodiesel has now attracted more and more attention due to its advantages in higher energy density and overall less greenhouse gas generation. Biodiesel (fatty acid alkyl esters) is produced by chemically or enzymatically catalyzed transesterification of lipids from microbial cells, microalgae, oil crops, or animal fats. Currently, plant oils or waste cooking oils/fats remain the major source for biodiesel production via enzymatic route, but the production capacity is limited either by the uncertain supplement of plant oils or by the low or inconsistent quality of waste oils/fats. In the past decades, significant progresses have been made on synthesis of microalgae oils directly from CO2via a photosynthesis process, but the production cost from any current technologies is still too high to be commercialized due to microalgae's slow growth rate on CO2, inefficiency in photo-bioreactors, lack of efficient contamination control methods, and high cost in downstream recovery. At the same time, many oleaginous microorganisms have been studied to produce lipids via the fatty acid synthesis pathway under aerobic fermentation conditions, among them one of the most studied is the non-c...Continue Reading

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Citations

Mar 18, 2020·Critical Reviews in Food Science and Nutrition·Muhammad BilalHairong Cheng
Sep 24, 2019·Biotechnology and Bioengineering·Mercedes LlamasElia Tomás-Pejó
Sep 25, 2018·Current Opinion in Biotechnology·Holly EkasHal S Alper
Feb 18, 2021·FEMS Yeast Research·Dmitry Mamaev, Renata Zvyagilskaya

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Methods Mentioned

BETA
genetic modifications
electrophoresis
GTPase
genetic modification

Software Mentioned

Microbia
DuPont

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