Integrating Study on Qualitative and Quantitative Characterization of the Major Constituents in Shuanghuanglian Injection with UHPLC/Q-Orbitrap-MS and UPLC-PDA.

Journal of Analytical Methods in Chemistry
Gen XueXin Chai

Abstract

As a clinically effective traditional Chinese medicine injection (TCMI), Shuanghuanglian injection (SHLI) is widely used in the treatment of upper respiratory tract infection and pneumonia. However, the shortage of quality analysis is a limitation that remains to be improved in the clinical application of SHLI. In this study, taking advantage of ultra-high-performance liquid chromatography tandem Q-Exactive Orbitrap high-resolution mass spectrometry (UHPLC/Q-Orbitrap-MS), 31 chemical components (eight organic acids, eleven flavonoids, five iridoid glycosides, four phenylethanoid glycosides, and three lignans) in SHLI were characterized, among which 22 components were unambiguously identified by reference compounds. The brief prediction results of network pharmacology indicated that the 22 targeted components may have anti-inflammatory, antibacterial, antiviral, and immunomodulatory activities. Using multiwavelength switching method, the 22 targeted components were quantified by ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) after the methodological validation. Based on the successfully established method, the total content of 22 components in 20 batches of SHLIs was efficiently determined with...Continue Reading

References

Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Dec 23, 2011·Nature·Felix Cheung
Jul 23, 2013·Journal of Chromatographic Science·Lan LuanRuichao Lin
Nov 7, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yuan GaoYun Qi
Jul 16, 2015·Journal of Pharmaceutical and Biomedical Analysis·Hongyang SunShizhong Chen
Oct 30, 2018·Nucleic Acids Research·Sunghwan KimEvan E Bolton
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuanli XiaJunping Kou
Mar 9, 2019·Frontiers in Pharmacology·Runzhi ZhangKang Ning
Nov 26, 2019·Journal of Ethnopharmacology·Wei LiuHongli Jiang

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