Integration and expression of Sorghum C(4) phosphoenolpyruvate carboxylase and chloroplastic NADP(+)-malate dehydrogenase separately or together in C(3) potato plants(1)

Plant Science : an International Journal of Experimental Plant Biology
A BeaujeanB S. Sangwan-Norreel

Abstract

We have integrated two cDNAs expressing Sorghum photosynthetic phosphoenolpyruvate carboxylase (C(4)-PEPC) and NADP-malate dehydrogenase (cpMDH), two key enzymes involved in the primary carbon fixation pathway of NADP-malic enzyme-type C(4) plants, separately or together into a C(3) plant (potato). Analysis of the transgenic plants showed a 1.5-fold increase in PEPC and cpMDH activities compared to untransformed plants. Immunolocalization confirmed an increase at the protein level of these two enzymes in the transgenic plants and indicated that the Sorghum cpMDH was specifically addressed to the chloroplasts of potato mesophyll cells. However, integration of either or both of the cDNAs into the potato genome did not appear to significantly modify either tuber starch grain content or the rate of photosynthetic O(2) production compared to control untransformed plants. The low level of transgene expression probably explains the lack of influence on carbon metabolism and photosynthetic rates. This general observation suggests that some complex mechanism may regulate the level of production of foreign C(4) metabolism enzymes in C(3) plants.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 26, 1984·Nucleic Acids Research·M Bevan

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Citations

Jun 5, 2003·Archives of Biochemistry and Biophysics·Mitsue Miyao, Hiroshi Fukayama
Oct 9, 2002·The Plant Journal : for Cell and Molecular Biology·Thomas RademacherChristoph Peterhänsel

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