PMID: 4881370Jul 1, 1968Paper

Integration and induction of phage P22 in a recombination-deficient mutant of Salmonella typhimurium

Journal of Virology
J P Wing

Abstract

Phage P22 can integrate as prophage into a recombination-deficient (Rec(-)) strain of Salmonella typhimurium. At 37 C, the integration efficiency is only 10% that in Rec(+) infection, but at 25 C the efficiencies in Rec(-) and Rec(+) hosts are similar. Rec(-) lysogens cannot be induced by ultraviolet irradiation or by treatments with the chemical inducing agents streptonigrin or mitomycin C. Heat induction of Rec(-) cells lysogenic for a temperature-sensitive c(2) mutant (ts c(2)) is normal, showing that the Rec(-) cell has the machinery necessary for prophage excision. Ultraviolet irradiation of Rec(-) (ts c(2)) lysogens prior to heat induction does not prevent the formation of infective centers after temperature shift. Thus, the noninducibility of Rec(-) lysogens is not due to destruction of the prophage as a result of ultraviolet irradiation. Deoxyribonucleic acid-ribonucleic acid (RNA) hybridization experiments demonstrate that no increase in phage-specific RNA synthesis occurs after ultraviolet irradiation of a Rec(-) (c(+)) lysogen. The Rec(-) mutant appears to lack part of the mechanism required to destroy the phage repressor and allow the initiation of early phage functions such as messenger RNA synthesis. A similar con...Continue Reading

References

May 1, 1968·Journal of Bacteriology·J P WingH O Smith
Jan 28, 1967·Journal of Molecular Biology·I Hertman, S E Luria
Mar 1, 1966·Journal of Molecular Biology·M H Green
Feb 1, 1967·Virology·H O Smith, M Levine
Jul 1, 1964·Journal of Molecular Biology·A P NYGAARD, B D HALL
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·H O SMITH, M LEVINE
Dec 18, 1964·Science·M LEVINE, H O SMITH

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Citations

Sep 12, 1973·Molecular & General Genetics : MGG·K G HardyB G Spratt
Sep 1, 1969·Bacteriological Reviews·B W Holloway
Jun 1, 1978·Microbiological Reviews·M M Susskind, D Botstein
Apr 1, 1976·Journal of Virology·S I Tokuno, M Gough
Dec 28, 1970·Journal of Molecular Biology·D Botstein, M J Matz
Feb 5, 1975·Journal of Molecular Biology·K BotsteinC A Swanson

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